Assessment of Lipid Peroxidation and Activities of Antioxidant Enzymes in Phosphide- Powder Residue Exposed Rats
Ayobola A Iyanda*
Department of Chemical Pathology, College of Health Sciences, Ladoke Akintola University of Technology, Osogbo, Nigeria
- *Corresponding Author:
- Ayobola A Iyanda
Department of Chemical Pathology
College of Health Sciences
Ladoke Akintola University of Technology
E-mail: [email protected]
Received date: October 01, 2012; Accepted date: October 31, 2012; Published date: November 02, 2012
Citation: Iyanda AA (2012) Assessment of Lipid Peroxidation and Activities of Antioxidant Enzymes in Phosphide- Powder Residue Exposed Rats. J Drug Metab Toxicol 3:132. doi:10.4172/2157-7609.1000132
Copyright: © 2012 Iyanda AA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cowpea, Vigna unguiculata (L.) Walpers is an important edible legume crop, is a good source of vegetable protein for millions of people. The estimation of global post-harvest losses caused by insect damage is enormous; therefore to prevent such wastage the use of synthetic chemical pesticides to protect stored grains is widespread. In some cases pesticide contamination of fumigated grains has been documented. The aim of this study to establish if oxidative stress is induced in animals exposed to such treated/contaminated cowpea. Eighteen female Wistar rats divided equally into three groups were used for the study. The rats in the first group were fed untreated cowpea and served as control while the rats in the second and third groups received phosphide-powder residue contaminated and uncontaminated cowpea respectively. Blood was drawn by retro-orbital bleeding. Analysis of blood to determine levels of malondialdehyde and glutathione as well as the activities of serum catalase, glutathione peroxidase, glutathione reductase, glutathione S transferase and superoxide dismutase in these rats revealed that while the level of reduced glutathione and activities of antioxidant enzymes were significantly reduced (p<0.05), the levels of malondialdehyde and oxidized glutathione were significantly increased (p<0.05) in rats fed with contaminated cowpea compared with control. These indices were not significantly changed (p>0.05) in rats fed with uncontaminated cowpea compared with control. The results of this study support the hypothesis that exposure of an animal to phosphide powder residue is capable of inducing oxidative stress.