Assessment of the Expression of Long Noncoding Mitochondrial RNAs (lncmtRNAs) During Cervical Cancer Progression and Cervical Carcinoma
|Komal Dadlani1, Constanza Lopez1, Fernando Gabler3, Juan Carlos Roa4, Claudio Villota1,2, Luisa Lina-Villa5, Enrique Boccardo6, Eva Bustamante7, Veronica Burzio1,2, Luis O. Burzio1,2 and Jaime Villegas Olavarria1,2*|
|1Andes Biotechnologies S.A, Science and Life Foundation, Santiago, Chile|
|2School of Biological Sciences, Andres Bello National University, Santiago, Chile|
|3Anatomy- Pathology Unit, Hospital San Borja Arriaran, Faculty of Medicine, University of Chile, Chile|
|4Department of Pathology, Catholic University of Chile, Santiago, Chile|
|5Faculty of Medicine, University of Sao Paulo, The Sao Paulo State Cancer Institute, Sao Paulo, Brazil|
|6Department of Microbiology, Institute of Biomedical Sciences, University of Sao Paulo, Sao Paulo, Brazil|
|7Oncology Clinic Foundation Arturo Lopez Perez, Rancagua, Santiago, Chile|
|*Corresponding Author :||Olavarria JV
Andes Biotechnologies S.A.-Science and Vida Foundation
Zanartu 1482-Nunoa, Santiago, Chile
Tel: + 56-2-23672097
E-mail: [email protected]
|Received: January 12, 2016 Accepted: February 16, 2016 Published: February 18, 2016|
|Citation: Dadlani K, Lopez C, Gabler F, Roa JC, Villota C, et al. (2016) Assessment of the Expression of Long Noncoding Mitochondrial RNAs (lncmtRNAs) During Cervical Cancer Progression and Cervical Carcinoma. J Cancer Sci Ther 8:038-045. doi:10.4172/1948-5956.1000386|
|Copyright: © 2016 Dadlani K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
Objective: Cervical cancer is the second most common cancer in women with high rates of mortality worldwide. Cervical cancer is slowly progressive and is preceded by pre-invasive intraepithelial lesions. Therefore, detection of premalignant lesions is key to preventing disease progression to advanced stages. The objective of the present study was examination and quantification of the differentially expressed non-coding mitochondrial RNAs during progression of disease.
Material and methods: The differential expression of S-ncmtRNA and AS-ncmtRNA was analyzed by in situ hybridization (ISH) using tissue macroarrays (TMA) from normal, CIN1, CIN2, CIN3 and invasive squamous carcinoma (SCC). PCNA and p16INK-4a were detected in consecutive biopsies by immunohistochemistry. Quantification of ISH signal was carried out with Image ProPlus 6.1 software and the results were expressed as percentage of Integrated Optical Density (IOD).
Results: We found a marked down-regulation of AS-ncmtRNA in 95% of tissues analyzed (CIN 1/2, CIN 3, and invasive squamous cancer). Moreover, differential expression of ASncmtRNA v/s S-ncmtRNA showed significant difference. Normal proliferating tissues did not display down-regulation of AS-ncmtRNA. Down regulation of ASncmtRNA correlated with the expression of the tumor suppressor protein p16INK-4a Conclusions: We found down-regulation of AS-ncmtRNA in pre-malignant and tumor samples which could distinguish normal tissues from early lesions and tumor samples. These results suggest that the down-regulation of AS-ncmtRNA is a novel marker of early lesions and cervix neoplasia.