Assessment of the Genetic Diversity, Breeds Structure and Genetic Relationships in Four Egyptian Camel Breeds using Microsatellite and Start Codon Targeted (SCoT) Markers
Received Date: May 22, 2018 / Accepted Date: Jul 18, 2018 / Published Date: Jul 28, 2018
The genetic diversity, relationships and population structure of sixty Egyptian camels derived from four breeds (Baladi, Sudani, Somali, and Maghrabi) were investigated using 18 microsatellite (SSRs) loci. In addition, the four breeds were genotyped using 16 Start Codon Targeted (SCoT) primers. A total of 346 SSR alleles were detected across the four camel breeds with an overall mean of 9.3 ± 0.66 alleles / locus. The mean number of alleles (MNA) and effective number of alleles (Ne) ranged from 9.2 ± 1.45 in the Baladi to 9.5 ± 1.27 in the Maghrabi breeds and from 6.5 ± 0.82 in the Maghrabi to 7.1 ± 0.93 in the Somali breeds, respectively. The values of observed heterozygosity (HObs) and expected heterozygosity (HExp) per breed varied from 0.82 ± 0.07 in the Maghrabi to 0.87 ± 0.07 in the Sudani camel breeds, and from 0.75 ± 0.03 in the Sudani to 0.79 ± 0.03 in the Maghrabi breeds , respectively. The genetic diversity estimated as the Shannon's information index (I) revealed the highest value (1.88 ± 0.14) in the Maghrabi and the lowest value (1.78 ± 0.18) in the Sudani breed. The values for fixation indices (FIS, FST and FIT) were -0.07284, 0.12364 and 0.05981, respectively. Thus indicating a moderate level of differentiation among the four breeds and a random mating process within each breed. The genetic structure revealed that the three breeds (Baladi, Sudani and Maghrabi) were genetically distinct and look like pure breeds, while the Somali breed showed some degree of admixture. A total of 153 amplicons were generated by the 16 SCoT primers, with an average of 9.56 amplicon/ primer and a polymorphism rate of 49%. The phylogenetic tree based on microsatellite and SCoT markers revealed that Maghrabi was separated in one cluster while, the second cluster comprised two sub-clusters. Sudani and Somali formed one sub-cluster and Baladi was in the second sub-cluster. Thus, the closest phylogenetic relationship was between the Sudani and Somali breeds.
Keywords: Genetic diversity; Population structure; Phylogenetic tree; Animal Genetic Resources (AnGR); Microsatellite; Start Codon Targeted (SCoT)
Citation: Al-Soudy A, El-Sayed A, El-Itriby HA, Hussein EHA (2018) Assessment of the Genetic Diversity, Breeds Structure and Genetic Relationships in Four Egyptian Camel Breeds using Microsatellite and Start Codon Targeted (SCoT) Markers. J Biodivers Endanger Species 6:001. Doi: 10.4172/2332-2543.S2-001
Copyright: © 2018 Al-Soudy, et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Select your language of interest to view the total content in your interested language
Share This Article
- Total views: 1606
- [From(publication date): 0-2018 - Nov 22, 2019]
- Breakdown by view type
- HTML page views: 1475
- PDF downloads: 131