alexa Assessment of the Genetic Diversity, Breeds Structure and Genetic Relationships in Four Egyptian Camel Breeds using Microsatellite and Start Codon Targeted (SCoT) Markers| Abstract
ISSN: 2332-2543

Journal of Biodiversity & Endangered Species
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  • Research Article   
  • J Biodivers Endanger Species S2: 001,
  • DOI: 10.4172/2332-2543.S2-001

Assessment of the Genetic Diversity, Breeds Structure and Genetic Relationships in Four Egyptian Camel Breeds using Microsatellite and Start Codon Targeted (SCoT) Markers

Al-Soudy A1*, El-Sayed A2,3, El-Itriby HA1 and Hussein EHA4
1Department of Animal Genetic Resources, National Gene Bank, Agricultural Research Center, Giza, Egypt
2Department of Animal Production, Faculty of Agriculture, Cairo University, Giza, Egypt
3Cairo University Research Park, Faculty of Agriculture, Cairo University, Giza, Egypt
4Department of Genetics, Faculty of Agriculture, Cairo University, Egypt
*Corresponding Author : Al-Soudy A, Department of Animal Genetic Resources, National Gene Bank, Agricultural Research Center, Giza, Egypt, Tel: 01021931386, Email: [email protected]

Received Date: May 22, 2018 / Accepted Date: Jul 18, 2018 / Published Date: Jul 28, 2018

Abstract

The genetic diversity, relationships and population structure of sixty Egyptian camels derived from four breeds (Baladi, Sudani, Somali, and Maghrabi) were investigated using 18 microsatellite (SSRs) loci. In addition, the four breeds were genotyped using 16 Start Codon Targeted (SCoT) primers. A total of 346 SSR alleles were detected across the four camel breeds with an overall mean of 9.3 ± 0.66 alleles / locus. The mean number of alleles (MNA) and effective number of alleles (Ne) ranged from 9.2 ± 1.45 in the Baladi to 9.5 ± 1.27 in the Maghrabi breeds and from 6.5 ± 0.82 in the Maghrabi to 7.1 ± 0.93 in the Somali breeds, respectively. The values of observed heterozygosity (HObs) and expected heterozygosity (HExp) per breed varied from 0.82 ± 0.07 in the Maghrabi to 0.87 ± 0.07 in the Sudani camel breeds, and from 0.75 ± 0.03 in the Sudani to 0.79 ± 0.03 in the Maghrabi breeds , respectively. The genetic diversity estimated as the Shannon's information index (I) revealed the highest value (1.88 ± 0.14) in the Maghrabi and the lowest value (1.78 ± 0.18) in the Sudani breed. The values for fixation indices (FIS, FST and FIT) were -0.07284, 0.12364 and 0.05981, respectively. Thus indicating a moderate level of differentiation among the four breeds and a random mating process within each breed. The genetic structure revealed that the three breeds (Baladi, Sudani and Maghrabi) were genetically distinct and look like pure breeds, while the Somali breed showed some degree of admixture. A total of 153 amplicons were generated by the 16 SCoT primers, with an average of 9.56 amplicon/ primer and a polymorphism rate of 49%. The phylogenetic tree based on microsatellite and SCoT markers revealed that Maghrabi was separated in one cluster while, the second cluster comprised two sub-clusters. Sudani and Somali formed one sub-cluster and Baladi was in the second sub-cluster. Thus, the closest phylogenetic relationship was between the Sudani and Somali breeds.

Keywords: Genetic diversity; Population structure; Phylogenetic tree; Animal Genetic Resources (AnGR); Microsatellite; Start Codon Targeted (SCoT)

Citation: Al-Soudy A, El-Sayed A, El-Itriby HA, Hussein EHA (2018) Assessment of the Genetic Diversity, Breeds Structure and Genetic Relationships in Four Egyptian Camel Breeds using Microsatellite and Start Codon Targeted (SCoT) Markers. J Biodivers Endanger Species 6:001. Doi: 10.4172/2332-2543.S2-001

Copyright: © 2018 Al-Soudy, et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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