Asymptomatic Malaria in School Children and Evaluation of the Performance Characteristics of the Partec Cyscope in the Mount Cameroon RegionHelen Kuokuo Kimbi1*, Hilda Uforka Ajeagah1, Frederick Chi Keka1, Emmaculate Lum1, Hervé Nyabeyeu Nyabeyeu2, Calvin Fotsing Tonga1, Asaah Humphrey Gah2 and Leopold Gustave Lehman2
- *Corresponding Author:
- Helen K Kimbi
Department of Zoology and Animal Physiology
Faculty of Science, University of Buea
P.O. Box 63, Buea, SWR, Cameroon
E-mail: [email protected]
Received date: September 10, 2012; Accepted date: September 26, 2012; Published date: September 30, 2012
Citation: Kimbi HK, Ajeagah HU, Keka FC, Lum E, Nyabeyeu HN, et al. (2012) Asymptomatic Malaria in School Children and Evaluation of the Performance Characteristics of the Partec Cyscope® in the Mount Cameroon Region. J Bacteriol Parasitol 3:153. doi: 10.4172/2155-9597.1000153
Copyright: ©2012 Kimbi HK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The key to the effective management of malaria is prompt and accurate diagnosis followed by effective treatment. The aim of this study was to determine asymptomatic malaria parasite prevalence and density and evaluate the performance characteristics of the Partec CyScope® (fluorescent microscope) in school children in the Mount Cameroon region using light microscopy as a gold standard. A total of 541 pupils aged 4 – 16 years were recruited into the study. After recording demographic data on each child, capillary blood was collected for the preparation of thin and thick blood films for the assessment of parasite density and speciation respectively. Five μl of blood was placed on the dye-labeled portion of the slide, cover-slipped, incubated for 1 minute and observed under the CyScope® for parasites. Performance characteristics of CyScope® were calculated. The overall prevalence of malaria was 64.0% and 58.4% for light microscopy and Partec CyScope® respectively. The overall geometric mean parasite density (GMPD) was 2255.22 (range 320-35040). The sensitivity of the test was 91.3% while the specificity was 86.7%. The Partec CyScope® showed a relatively high sensitivity and specificity in diagnosing malaria in school children and could therefore be used in mass surveillance programmes for the management and control of malaria.