alexa Attempt to Detect Garlic Allyl Sulphides from Saliva after Consumption of Garlic Tablets Using GC-MS | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Attempt to Detect Garlic Allyl Sulphides from Saliva after Consumption of Garlic Tablets Using GC-MS

Cathy J Watson1*, David de Souza2, Claudio Silva2, Dedreia Tull2, Suzanne M Garland3 and Larry L Lawson4

1General Practice and Primary Health Care Academic Centre, The University of Melbourne, Carlton, Australia

2Metabolomics Australia, Bio21 Institute, The University of Melbourne, Parkville, Australia

3Dept Microbiology Infectious Diseases, Royal Women’s Hospital, Department of Obstetrics and Gynaecology University of Melbourne, Carlton 3053, Australia

4Silliker Inc., Chicago, Illinois, USA

*Corresponding Author:
Cathy Watson
The University of Melbourne
General Practice and Primary Health Care
Academic Centre, 200 Berkeley St
Carlton, 3053, Australia
Tel: +61 3 9035 5685
Fax: +61 3 9347 6136
E-mail: [email protected]

Received date: July 29, 2013; Accepted date: August 21, 2013; Published date: August 24, 2013

Citation: Watson CJ, de Souza D, Silva C, Tull D, Garland SM, et al. (2013) Attempt to Detect Garlic Allyl Sulphides from Saliva after Consumption of Garlic Tablets Using GC-MS. J Microb Biochem Technol 5:081-083. doi:10.4172/1948-5948.1000104

Copyright: © 2013 Watson CJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Allicin is an organosulfur compound of Allium sativum (garlic) with anti-fungal and antimicrobial properties. However, to date, neither allicin nor its metabolites has been detected in human organs or bodily fluids following oral consumption of garlic. Bioavailability has only been demonstrated using breath studies. Enteric-coated formulations are designed to pass through the acidic stomach environment; hence detection of garlic compounds in saliva following consumption of enteric-coated garlic tablets would provide evidence of bioavailability in a body secretion. An attempt was made to identify metabolites dially disulphide and allyl methyl sulphide using Gas Chromatography Mass Spectrometry (GC-MS). We completed a single participant mass spectrometry study using 13 time points over 24 hours following consumption of 20 enteric-coated garlic tablets. There was no detection of allicin derived sulfides at any time point. In summary, it is possible that these highly volatile compounds may be more readily detectable using a solid phase micro-extraction GC-MS, or headspace analysis methodology, although the less volatile allyl methyl sulfone and allyl methyl sulfoxide may be the main metabolites present.


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