AZT Treatment Increases mtDNA Mutations in HepG2 and CCD-1112Sk Cells
Adam E. Osborne*, John E. Rice, J Aquiles Sanchez and Lawrence J. Wangh
- *Corresponding Author:
- Adam E Osborne
Biology, Brandeis University
Waltham, MA, 02454, USA
Received Date: August 07, 2013; Accepted Date: September 29, 2013; Published Date: October 11, 2013
Citation: Osborne AE, Rice JE, Sanchez AJ, Wangh LJ (2013) AZT Treatment Increases mtDNA Mutations in HepG2 and CCD-1112Sk Cells. J AIDS Clin Res 4:250. doi:10.4172/2155-6113.1000250
Copyright: © 2013 Osborne AE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mitochondrial dysfunction is linked to disease, but it remains unclear whether accumulation of random mutations in the mitochondrial genome is the cause of dysfunction. Using digital or near-digital LATE-PCR with Lights-On/Lights- Off probes we have measured the mutational load in mitochondrial genomes. Exposure of HepG2 and CCD-1112Sk cells to AZT for thirty days caused a significant increase in mutations in the three mitochondrial loci examined. These results demonstrate the utility of our method for analysis of mutational load without sequencing and reinforce the fact that mitochondrial DNA damage due to drugs, aging, and disease should be studied in detail.