alexa Bacterial Etiology of Diarrhoeal Disease in Children and Antibiogram of the Isolates | OMICS International | Abstract
ISSN: 2327-5073

Clinical Microbiology: Open Access
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Research Article

Bacterial Etiology of Diarrhoeal Disease in Children and Antibiogram of the Isolates

Anjeeta Neupane1*, Pramila Parajuli1, Rama Bastola1 and Anjan Paudel2

1Department of Microbiology, St. Xavier’s College, Kathmandu, Nepal

2Faculty of Pharmacy, Jamia Hamdard, New Delhi, India

*Corresponding Author:
Anjeeta Neupane
St. Xavier’s College, Tribhuvan University
Kathmandu, Nepal
Tel: +9779845150048
E-mail: [email protected]

Received date: April 11, 2017; Accepted date: April 24, 2017; Published date: April 30, 2017

Citation: Neupane A, Parajuli P, Bastola R, Paudel A (2017) Bacterial Etiology of Diarrhoeal Disease in Children and Antibiogram of the Isolates. Clin Microbiol 6:278. doi:10.4172/2327-5073.1000278

Copyright: © 2017 Neupane A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Objective: The objective of this study was to determine the bacterial etiology of diarrhoeal disease in children and the antibiogram of the isolates.

Methods: The study was carried out in Bharatpur Hospital, Chitwan, Nepal form May 2014 to Oct 2014. Stool specimens were collected from children at paedriatic ward aseptically and were processed in the Microbiology laboratory. Each sample was processed macroscopically and microbiologically. The culture of the specimen and identification of the isolates were done as per the standard guidelines and results were interpreted on the basis of different tests.

Results: Out of 202 specimens, 84 (42%) samples showed growth and 118 (58%) samples presented no any growth. Highest number of growth was observed in samples from male patients than from female patients. The predominant pathogen was Aeromonas species 33 (12%), followed by NLF E. coli 19 (6.9%), Proteus mirabilis 14 (5.1%). Amikacin (94%) was the most effective antibiotic and the least effective antibiotic was Amoxy-clavulanic acid (6%). In this research, the highest multidrug resistant organism was Aeromonas species. There was significant association between diarrhoea and treatment of water (p<0.05).

Conclusion: Thus, bacterial etiology of diarrhoeal disease in children and antibiogram of the isolates were determined, which was found to be an important diagnostic test for the diarroheal patient. From this study, research investigation were shown significant association between diarrhea and treatment of water, hand washing, signs and symptoms like fever, loss of appetite, abdominal cramps. Thus the study might help in the assessment of the current anti-microbial resistance patterns of bacterial isolates in diarrheal infection and helps in the formulation of the strategies to reduce the infection rate in Bharatpur Hospital.


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