Bartonella Henselae Initial Infection of Mature Human Erythrocytes Observed in Real Time Using Bacterial Endogenous Fluorescence
Gislaine Vieira-Damiani1,2*, Marna Elise Ericson3, Marilene Neves da Silva1, Kalpna Gupta4, Tania Benetti Soares1, Amanda Roberta de Almeida1, Vitor Bianchin Pelegati5, Mariana Ozello Baratti5, Carlos Lenz Cesar5, Maria Letícia Cintra6 and Paulo Eduardo Neves Ferreira Velho1
- *Corresponding Author:
- Gislaine Vieira-Damiani
State University of Campinas (UNICAMP)
Medical Sciences School
Rua Tessália Vieira de Camargo
126 - Cidade Universitária“Zeferino Vaz”
CEP: 13083-887 - Campinas – SP, Brasil
Tel/ Fax: (55) 19 3521 9134
E-mail: [email protected]
Received Date: January 24, 2016; Accepted Date: February 13, 2016; Published Date: February 20, 2016
Citation: Vieira-Damiani G, Ericson ME, da Silva MN, Gupta K, Soares TB, et al. (2016) Bartonella Henselae Initial Infection of Mature Human Erythrocytes Observed in Real Time Using Bacterial Endogenous Fluorescence. J Trop Dis 4:207. doi:10.4172/2329-891X.1000207
Copyright: © 2016 Vieira-Damiani G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bartonella henselae is a causative agent of anemia, cat scratch disease, bacillary angiomatosis, recurrent fever, hepatitis, endocarditis, chronic lymphadenopathy, joint and neurological disorders. B. henselae are intra-erythrocytic bacteria. The goal of this study was to visualize the B. henselae invasion into enucleated human red blood cells in real time using bacterium endogenous fluorescence. We took advantage of the unique fluorescence emission spectral profile of the bacteria. We used a linear unmixing approach to separate the fluorescence emission spectra of human erythrocytes from native B. henselae when excited at 488nm. Human blood samples were inoculated with B. henselae and incubated for 60 hours. 3-D live images were captured at select intervals using multi-photon laser scanning microscopy. Uninfected blood samples were also analyzed. This study revealed bacteria entering mature erythrocytes over a 60 hour time period.