alexa Bench Top Bioassay of Anti-tumor Activity of Black Cumin (Nigella sativa) Oil and Bee Honey Using the Indigenous Strain of Agrobacterium tumefaciens SDB0012

Journal of Research and Development
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Bench Top Bioassay of Anti-tumor Activity of Black Cumin (Nigella sativa) Oil and Bee Honey Using the Indigenous Strain of Agrobacterium tumefaciens SDB0012

Alseed HAMG1, Elkedir AEE2* and Ahmed RAM3

1Center of Biosciences and Biotechnology, Faculty of Engineering and Technology, University of Gezira, Dubai

2Industrial Research and Consultancy Center, Khartoum, Sudan

3The National Ribat University Faculty of Pharmacy, Khartoum, Sudan

*Corresponding Author:
Elkedir AEE
Industrial Research and Consultancy Center
Khartoum, Sudan
Tel: +00249912490610
E-mail: [email protected]

Received Date: February 17, 2016 Accepted Date:March 19, 2016 Published Date: March 30, 2016

Citation:Alseed HAMG, Elkedir AEE and Ahmed RAM (2016) Bench Top Bioassay of Anti-tumor Activity of Black Cumin (Nigella sativa) Oil and Bee Honey Using the Indigenous Strain of Agrobacterium tumefaciens “SDB0012”. J Res Development 4: 143. doi: 10.4172/2311-3278.1000143

Copyright: © 2016 Alseed HAMG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Sudan is endowed with natural products having anti-bacterial, anti-fungal and anti-cancer activities. Among these, bee honey and black cumin oil are recommended by botanist to be used as antibacterial and anti-cancer products; in addition to other health uses. In this study both bee honey, crude oil of black cumin and a mixture of them at a rate of 1:1 were studied for anti-bacterial and anti-tumor activities using the potato disc bioassay at the Microbiology Laboratory, Faculty of Engineering and Technology, University of Gezira. The indigenous strain SDB0012 used in this study was extracted from Tuti Island-Khartoun State and purified since 2005. The major characteristic of this strain was given by Yousif. Whereas, bee honey and black cumin oil were collected in Wad Medani market. Moreover, the crude oil of the black cumin was subjected to fractionation using thin layer chromatography and potato disc bioassay to identify the active anti-tumor components in these trials, the Complete Randomized Design was used with four replicates. Results indicated that both bee honey and the crude extract of the black cumin had no anti-bacterial activities against the indigenous strain of Agrobacterium tumefaciens “SDB0012”, which characterized by having resistance against penicillin (30 mg), chloramphenicol (30 mg) and ciprofloxacin (30 mg). Both bee honey and black cumin had anti-tumor activities with inhibition of 40% and 55%, respectively. The mixture of bee honey and cumin oil extract (1:1) gave 100% inhibition of tumor on the potato discs. Only one out of the eight components separated from the oil black cumin crude extract in thin layer chromatography showed complete inhibition of tumors and was considered as the active anti-tumor component of the black cumin.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version