Bio Control Potential of Pseudomonas fluorescens against Coleus Root Rot Disease
- *Corresponding Author:
- Ramjegathesh R
Department of Plant Pathology, Centre for Plant Protection Studies
Tamil Nadu Agricultural University
Coimbatore-641 003, Tamil Nadu, India
E-mail: [email protected]
Received date: December 27, 2013; Accepted date: January 17, 2014; Published date: January 24, 2014
Citation: Vanitha S, Ramjegathesh R (2014) Bio Control Potential of Pseudomonas fluorescens against Coleus Root Rot Disease. J Plant Pathol Microb 5:216. doi:10.4172/2157-7471.1000216
Copyright: © 2014 Vanitha S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ten different strains of Pseudomonas fluorescens were isolated from coleus rhizosphere except the pf1 strain and identified by biochemical tests. These strains were screened against Macrophomina phaseolina (Tassi) Goid, the causal organism of coleus root rot. The results revealed that Pf1 strains recorded maximum inhibition of mycelial growth against control. The mechanism of Pseudomonas strains namely the iron-chelating agent (siderophore), volatiles (HCN) and antibiotic (Fluorescein and pyocyanin) production tests were studied and reacted for siderophore, antibiotic and HCN production. The talc-based formulation of Pf1 and CPF1 was prepared and the bio-efficacy was tested under greenhouse conditions. The stem cutting and soil application of the talc-based formulation of Pf1 significantly reduced the root rot incidence and increased shoot and tuber length also.