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Bioactive Collagen Membrane as a Carrier for Sustained Release of PDGF | OMICS International | Abstract
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

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Research Article

Bioactive Collagen Membrane as a Carrier for Sustained Release of PDGF

Seiichi Yamano1*, Terry Y. Lin1, Jisen Dai1, Kathryn Fabella1 and Amr M. Moursi2
1Department of Prosthodontics, New York University College of Dentistry, 345 East 24th Street, New York, NY 10010, USA
2Department of Pediatric Dentistry, New York University College of Dentistry, New York, NY 10010, USA
Corresponding Author : Dr. Seiichi Yamano
Department of Prosthodontics
New York University College of Dentistry
345 East 24th Street, 4W, New York
NY 10010, USA
Tel: 212-998-9714
Fax: 212-992-7100
E-mail: [email protected]
Received October 27, 2011; Accepted November 16, 2011; Published November 18, 2011
Citation: Yamano S, Lin TY, Dai J, Fabella K, Moursi AM (2011) Bioactive Collagen Membrane as a Carrier for Sustained Release of PDGF. J Tissue Sci Eng 2:110. doi: 10.4172/2157-7552.1000110
Copyright: © 2011 Yamano S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The objective of this study was to evaluate the ability of a collagen membrane (CM) as a carrier to successfully deliver platelet-derived growth factor (PDGF) and to observe the subsequent effects of the factor on preosteoblasts in vitro. MC3T3-E1 mouse preosteoblasts were cultured with a commercially available CM containing PDGF. After a two-day cell culture, cell viability was investigated by the MTT assay and cell proliferation was assessed by the crystal violet proliferation assay. Expression levels of the following osteoblastic differentiation marker genes were measured by real-time PCR: runt-related transcription factor 2 (RUNX2), osteopontin (OPN), bone sialoprotein (BSP), and osteocalcin (OCN). A cell proliferation assay was conducted, and osteoblastogenesis was determined by alkaline phosphatase (ALP) activity. A sustained release of PDGF from a CM was observed for ~3 weeks. Gene expression of all RUNX2, OPN, BSP, and OCN in CM with PDGF was significantly upregulated compared to those in CM without PDGF (all p < 0.05). Interestingly, CM without PDGF also significantly increased gene expression of RUNX2 and OPN in MC3T3-E1 cells compared to the cell control (both p < 0.05). Furthermore, it was observed that the PDGF released from CM significantly promoted ALP activity and cell proliferation with little cytotoxicity. These results suggest that a CM can be utilized for sustained delivery of PDGF. Also, released PDGF can promote MC3T3-E1 cell activities. This strategy may lead to an improvement in the current clinical treatment of bone defects in periodontal and implant therapy.

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