Bioactivity from IndonesianÃ¢ÂÂs Marine Sponge Xestospongia Sp. as Antibacterial Resistance Escherichia coli
Khairunnisa and Viqqi Kurnianda*
Department Marine Science, Faculty Marine and Fisheries, Syiah Kuala University, Indonesia
- *Corresponding Author:
- Viqqi Kurnianda
Department Marine Science, Faculty
Marine and Fisheries, Syiah Kuala University
E-mail: [email protected], [email protected]
Received date: April 19, 2017; Accepted date: April 25, 2017; Published date: May 03, 2017
Citation: Khairunnisa, Kurnianda V (2017) Bioactivity from Indonesian’s Marine Sponge Xestospongia Sp. as Antibacterial Resistance Escherichia coli. Nat Prod Chem Res 5: 265. doi: 10.4172/2459-6836.1000265
Copyright: © 2017 Khairunnisa, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The isolation and characterization of bioactive compounds of Indonesian’s marine sponge Xestospongia sp. has been done. Bioactive secondary metabolites alkaloid was isolated based on bioassay guided-separation with several steps of chromatography and monitored by the inhibition of antibacterial test of Escherichia coli. V15B02 compound is identified as the alkaloid compounds at Rf value of 0.7 using a mobile phase of methanol: water 9:1 (v/v). FTIR spectrum data interpretation results showed that the compound of V15B02 has hydroxyl groups with their O-H stretching vibration in the area around 3425.58 cm-1 and C-N vibration in the fingerprint region around 1111.00 cm-1. Their sharp band with sharp and strong intensity on the number of 2924.09 cm-1 is an aliphatic C-H. That this was reinforced by a sharp uptake and up down in numbers of 1404.18 cm-1 which is C-H band indicates the terminal methyl groups. In the catchment area 1636.64 cm-1 showed the presence of C=O group. Indication of a cyclic amine group of alkaloids visible from the drop of vibration tertiary N in the area around 1165.00 cm-1. V15B02 compounds have an activity inhibiting the growth of bacterial resistance Escherichia coli at a concentration of 70 ug/mL.