alexa Bioanalytical Method Development and Validation for Herbal Quercetin in Nano Formulation by RP-UFLC in Rabbit Plasma
ISSN: 0975-0851

Journal of Bioequivalence & Bioavailability
Open Access

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Bioanalytical Method Development and Validation for Herbal Quercetin in Nano Formulation by RP-UFLC in Rabbit Plasma

Shanmugam R1*, Gowthamarajan K1*, Priyanka DL1, Madhuri K2 and Narayanareddy Karri VVS1

1Department of Pharmaceutics, JSS College of Pharmacy (A constituent college of JSS University, Mysore), Udhagamandalam, The Nilgiris, Tamilnadu, India

2Department of Pharmacology, JSS College of Pharmacy (A constituent college of JSS University, Mysore), Udhagamandalam, The Nilgiris, Tamilnadu, India

*Corresponding Author:
Shanmugam R
Research Scholar, Department of Pharmaceutics
JSS College of Pharmacy, Rocklands
Udhgamandalam-643001, The Niligiris
Tamilnadu, India
Tel: +91-9843454943
Fax: 0423-2442937
E-mail: [email protected]

Gowtahmarajan K
Department of Pharmaceutics
JSS College of Pharmacy
Rocklands, Udhgamandalam-643001
The Niligiris, Tamilnadu, India
Tel: +91-9443089812
Fax: 0423-2442937
E-mail: [email protected]

Received Date: July 03, 2013; Accepted Date: July 18, 2013; Published Date: July 26, 2013

Citation: Shanmugam R, Gowthamarajan K, Priyanka DL, Madhuri K, Narayanareddy Karri VVS (2013) Bioanalytical Method Development and Validation for Herbal Quercetin in Nano Formulation by RP-UFLC in Rabbit Plasma. J Bioequiv Availab 5:191-196. doi: 10.4172/jbb.1000157

Copyright: © 2013 Shanmugam R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A sensitive reverse phase ultra fast liquid chromatography (RP-UFLC) has been developed and validated for the quantification of Quercetin in nano formulation in rabbit plasma using solid phase extraction method (SPE). Best chromatographic resolution was achieved on a reverse phase Hibar®C18 (250×4.6 mm i.d., 5 μ) column with the mobile phase consisted of acetonitrile: pottasium dihydrogen ortho phosphate (pH 3.5) in the ratio of 65:35 (v/v) with a flow rate of 0.8 mL/min. The retention time for the drugs was found to be 8.6 min and 10.0 min for internal standard (fluticasone propionate). The analyte was detected by using a Photo-diode array (PDA) detector with LC solution software. Linearity was obtained in the concentration range of 10 to 400.0 ng/mL (r2= 0.989). Lower limit of quantification (LLOQ) was found to be 10 ng/mL. Middle limit of quantification (MQC) was found to be 200 ng/mL and higher limit of quantification (HQC) was found to be 380 ng/mL. The average recovery of the analyte was found to ranging from 95.91 to 98.59%. The developed method isapplicablefor estimation of Quercetin in nano-formulation, routine quality control analysis, pharmacokinetic and bioequivalence studies.

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