alexa
Reach Us +18023424982
Bioassay of <em>Toxoplasma Gondii</em> from Apparently Healthy Pigs Slaughtered in Addis Ababa Abattoir, Ethiopia | OMICS International | Abstract
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
All submissions of the EM system will be redirected to Online Manuscript Submission System. Authors are requested to submit articles directly to Online Manuscript Submission System of respective journal.

Research Article

Bioassay of Toxoplasma Gondii from Apparently Healthy Pigs Slaughtered in Addis Ababa Abattoir, Ethiopia

Endrias Zewdu Gebremedhin1*, Mulisa Megerssa Kebeta2, Mebratu Asaye3, Hagos Ashenafi4, Vincenzo Di Marco5 and Maria Vitale5

1Department of Veterinary Laboratory Technology, Faculty of Agriculture and Veterinary Science, Ambo University, P.O. Box 19, Ambo, Ethiopia

2College of Veterinary Medicine, Jigjiga University, Jijiga, Ethiopia

3Faculty of Veterinary Medicine, Gondar University, Gondar, Ethiopia

4Department of Parasitology and Pathology, College of Veterinary Medicine and Agriculture, Addis Ababa University, Debre Zeit, Ethiopia

5Italian National Reference Centre for Toxoplasmosis at Istituto Zooprofilattico Sperimentale della Sicilia A. Mirri, Italy

*Corresponding Author:
Endrias Zewdu Gebremedhin
Department of Veterinary Laboratory Technology
Faculty of Agriculture and Veterinary Science, Ambo
University, P.O. Box 19, Ambo, Ethiopia
Tel: +251-911894802
E-mail: [email protected]

Received date: August 17, 2015 Accepted date: September 10, 2015 Published date: September 12, 2015

Citation: Gebremedhin EZ, Kebeta MM, Asaye M, Ashenafi H, Marco VD, et al. (2015) Bioassay of Toxoplasma Gondii from Apparently Healthy Pigs Slaughtered in Addis Ababa Abattoir, Ethiopia. J Veterinar Sci Technol 6:258. doi:10.4172/2157-7579.1000258

Copyright: © 2015 Gebremedhin, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Toxoplasmosis is an infectious disease caused by the protozoan parasite Toxoplasma gondii that infects a wide range of animals, including man and birds. The objective of the study was to isolate T. gondii in pigs slaughtered at the Addis Ababa abattoir. A total of 290 sera were examined for T. gondii antibodies (IgG) using the Direct Agglutination Test (DAT) of which 111 pigs (38.28%; 95% confidence interval [CI]: 32.65%-44.14%) were seropositive. Hearts of fifty seropositive pigs and two pooled pig heart samples (portion of 4 pig hearts per pool) were used for the study. For the isolation of T. gondii, 50 g heart tissue was ground, digested with acid-pepsin and inoculated intraperitoneally into mice (5 mice/sample). Viable T. gondii was isolated from hearts of 24 of 50 (48%; 95% CI: 33.7%-62.6%) seropositive pigs and from the two pooled seronegative pig hearts. All isolates were asymptomatic except one isolate from Bishoftu which was lethal for mice on day 28 post-inoculation. There was a perfect agreement (k=0.85) between DAT and microscopic cyst detection in mice. The study suggests that pork containing viable T. gondii is released onto markets for consumers. Therefore, enforcing hygienic measures, public education, provision of rendering facilities and further studies deserve consideration. This is the first report of isolation of viable T. gondii from pigs in Ethiopia.

Keywords

Top