Bioassay-guided Isolation of a Antinociceptive, Anti-inflammatory and Antipyretic Benzofuran Derivative from Viburnum grandiflorumGhias Uddin1*, Muhammad Alam1, Naveed Muhammad2, Bina S Siddiqui3 and Anwar Sadat1
- *Corresponding Author:
- Ghias Uddin
Institute of Chemical Sciences
Centre for Phytomedicine and Medicinal Organic Chemistry
University of Peshawar, Peshawar, Pakistan
E-mail: [email protected]; [email protected]
Received date: October 08, 2013; Accepted date: November 26, 2013; Published date: November 28, 2013
Citation: Uddin G, Alam M, Muhammad N, Siddiqui BS, Sadat A (2013) Bioassayguided Isolation of a Antinociceptive, Anti-inflammatory and Antipyretic Benzofuran Derivative from Viburnum grandiflorum. Pharmaceut Anal Acta 4:274. doi: 10.4172/2153-2435.1000274
Copyright: © 2013 Uddin G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In tradition system of medicines the Vibernum grandiflorum is widely practiced as antipyretic in the treatment of malaria and typhoid. The current study was designed for scientific validation of antinociceptive, anti-inflammatory and antipyretic effect of V. grandiflorum with reference to its chemical composition. The crude ethanolic extract and isolated compound was tested for its antinociceptive effect using acetic acid and hot plate pain models. The anti-inflammatory potential was investigated through carrageenan induced oedema and antipyretic effect was determined using brewer’s yeast induced pyrexia. Beside the isolation of β-sitosterol, ursolic acid and betulin, a benzofurane derivative, 2-(-4’-hydroxy-3’-methoxy-phenyl)-5-(3”-hydroxy-propyl)-3-hydroxy-methyl-7-hydroxy-2, 3-dihydrobenzofuran was first time isolated from the roots of V. grandiflorum. The crude extract and compound 1 were found to be active in protection of induced writhing (70.45 and 82.11%), inhibition of increase in paw volume (71.34 and 54.47%), and attenuation of pyrexia (71.78 and 41.68%). It is concluded that the significant inhibition of acetic acid induced writhing, protection of carrageenan induced paw oedema and attenuation of induced pyrexia by isolated compound 1 strongly supports the antinociceptive, anti-inflammatory and antipyretic effect of the roots of V. grandiflorum. This research work also provides scientific rationale to the folkloric use of V. grandiflorum as pain killer and antipyretic.