Biochemical Approach for Virulence FactorsÃ¢ÂÂ Identification in
Xanthomonas Oryzae Pv. Oryzae
Sylvestre Gerbert Dossa C1,2*, Petr Karlovsky1 and Kerstin Wydra3
1Molecular Phytopathology and Mycotoxin Research, University of Goettingen, Grisebachstr.6 37077, Germany
2International Rice Research Institute, Division of Plant Breeding, Genetics and Biochemistry, DAPO Box 7777, Metro Manila, Philippines
3Erfurt University of Applied Sciences, Altonaer Str. 25, 99085 Erfurt, Germany
- *Corresponding Author:
- Gerbert Sylvestre Codjo Dossa
Molecular Phytopathology and Mycotoxin Research
University of Goettingen, Grisebachstr.6 37077, Germany
E-mail: [email protected]
Received date: March 10, 2014; Accepted date: March 29, 2014; Published date: April 07, 2014
Citation: Sylvestre Gerbert Dossa C, Karlovsky P, Wydra K (2014) Biochemical Approach for Virulence Factors’ Identification in Xanthomonas Oryzae Pv. Oryzae. J Plant Pathol Microb 5:222. doi:10.4172/2157-7471.1000222
Copyright: © 2014 Sylvestre Gerbert Dossa C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bacterial blight caused by Xanthomonas oryzae pv. oryzae (Xoo) leads to a substantial yield reduction of up to 50% in most rice-growing regions. Host plant resistance is an effective control method, and more than 30 resistance genes have been identified in rice genotypes. To understand the interaction of the pathogen in a susceptible reaction of the host plant, Xoo culture filtrate and treated culture filtrates were used to treat two rice genotypes using four strains Mai1, PXO88, Dak1 and Dak16. The study revealed that Xoo culture filtrate, heated culture filtrate and proteinase K treated culture filtrate induced typical bacterial blight symptoms on rice genotypes IRBB4 and FKR14 with a maximum lesion length of about 23.1 cm for culture filtrate. Heated culture filtrate phytotoxicity effects on both rice genotypes was with highest lesion length of about 6.9 cm, while 13.4 cm was the maximum length induced by a proteinase K treated fraction. After ethyl acetate treatment of the culture filtrate, a considerable reduction of the phytotoxicity was observed. Therefore we suggest that a low molecular-weight toxin may be present in the ethyl acetate extract should not play a major role in Xoo virulence and speculate that EPS, Xylanase, polygalacturonase, proteinaceouse contribute to Xoo virulence.