alexa Biocompatibility Of Synthesised Nano-Porous Anodic Aluminium Oxide Membranes For Use As A Cell Culture Substrate For Madin-Darby Canine Kidneys Cells: A Preliminary Study
ISSN: 2157-7552

Journal of Tissue Science & Engineering
Open Access

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Research Article

Biocompatibility Of Synthesised Nano-Porous Anodic Aluminium Oxide Membranes For Use As A Cell Culture Substrate For Madin-Darby Canine Kidneys Cells: A Preliminary Study

GEJ Poinern1*, N Ali1, C Berry2, P Singh3, S Berchmans4 and D Fawcett1
1Murdoch Applied Nanotechnology Research Group, Department of Physics, Energy Studies and Nanotechnology, School of Engineering and Energy, Murdoch University, Murdoch, Western Australia 6150, Australia
2School of Veterinarian and Biomedical Sciences, Division of Health Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
3School of Chemical and Mathematical Sciences, Murdoch University, Murdoch, Western Australia 6150, Australia
4Electrodics and Electrocatalysis Division, Central Electrochemical Research Institute (CECRI-CSIR), Karaikudi630006, Tamil Nadu, India
Corresponding Author : Dr. Gerrard Eddy Jai Poinern
Murdoch Applied Nanotechnology Research Group
Department of Physics, Energy Studies and Nanotechnology
School of Engineering and Energy, Murdoch University
Murdoch, Western Australia 6150, Australia
Tel: +61 8 9360-2892
Fax: +61 8 9360-6183
E-mail: [email protected]
Received August 02, 2012; Accepted September 12, 2012; Published September 17, 2012
Citation:Poinern GEJ, Ali N, Berry C, Singh P, Berchmans S, et al. (2012) Biocompatibility of Synthesised Nano-Porous Anodic Aluminium Oxide Membranes for Use as a Cell Culture Substrate for Madin-Darby Canine Kidneys Cells: A Preliminary Study. J Tissue Sci Eng 3:119. doi: 10.4172/2157-7552.1000119
Copyright: © 2012 Poinern GEJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

In this study we investigate for the first time the biomedical potential of using a membrane made from anodic aluminium oxide (AAO) for culturing the Madin-Darby Canine Kidney (MDCK) epithelial cell line. Nano-porous aluminium oxide membranes exhibit interesting properties such as high porosity, which allows the exchange of molecules and nutrients across the membrane and can be made with highly specific pore sizes that can be preselected by adjusting the controlling parameters of a temperature controlled two-step anodization process. The cellular response and interactions of the MDCK cell line with the synthesised nano-porous AAO membrane, a commercially available membrane and a glass control were assessed by investigating cell adhesion, morphology and proliferation.

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