Bioconjugated Magnetic Nanoparticles for Rapid Capture of Gram-positive Bacteria
Longyan Chen and Jin Zhang*
Department of Chemical & Biochemical Engineering, University of Western Ontario, London, Ontario, N6A 5B9, Canada
- *Corresponding Author:
- Jin Zhang, Ph.D
Department of Chemical & Biochemical Engineering
University of Western Ontario
London ON. Canada N6A 5B9
Tel: (519) 661 2111; (519) 668 8322
E-mail: [email protected]
Received Date: September 22, 2012; Accepted Date: October 26, 2012; Published Date: October 28, 2012
Citation: Chen L, Zhang J (2012) Bioconjugated Magnetic Nanoparticles for Rapid Capture of Gram-positive Bacteria. J Biosens Bioelectron S11:005. doi: 10.4172/2155-6210.S11-005
Copyright: © 2012 Chen L, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this paper, bioconjugated Magnetic Nanoparticles (MNPs) are developed for rapid capture gram-positive bacterium Staphyloccocus aureus (S. aureus). The MNPs was synthesized through a two-step sol-gel process, followed a bioconjugation of gentamicin (Gm), an aminoglycoside antibiotic, via the linker, glutaraldehyde. The average diameter of the magnetic core is 18 ± 3 nm and the thickness of shell is around 5 ± 3 nm. The XRD results indicate that core-shell MNPs consist of magnetic core, Fe3O4, and silica (SiO2) shell. In addition, the core-shell MNPs show the ferromagnetic properties, whereas the monodipersed Iron Oxide Magnetic Nanoparticles (IONPs), which were produced in the first step, show the typical superparamagnetic properties with a blocking temperature (TB) at 115 K. The interactions between S. aureus and core-shell MNPs with and without Gm have been further investigated by using a Transmission Electron Microscopy (TEM). Our results demonstrate that the diluted S. aureus
with the concentration as low as 0.5 ×103 CFU/mL can be separated from the solution by the core-shell MNPs in one minute.