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Abstract
Biodegradation of Azo Dyes by Three Isolated Bacterial Strains: An Environmental Bioremedial Approach
The present study was conducted to investigate the decolorization and degradation of azo dyes using bacteria isolated from textile dye effluent. Three different bacterial species were isolated and the isolates were identified as Bacillus subtilis, Pseudomonas aeruginosa, and Psuedomonas putida. The bacterial inoculums were inoculated into flasks containing azo dyes (500 mg/l) with trace amounts of yeast extract, glucose and sucrose and then sterilized and incubated for 4 days. The decolourization was expressed in terms of percentage decolourization. Psuedomonas putida (95%) was identified as the best decolourizer of Blue RR. Pseudomonas aeruginosa (93%) was the best decolourizer of Black B. The best decolourizer of Red RR was Bacillus subtilis (91%). Bacillus subtillis (65%) highly decolourized the Yellow RR. Pseudomonas aeruginosa (70.58%) was the best decolourizer of Navy Blue. The degradation product after decolorization was examined by thin layer chromatography and Fourier transformed infrared spectroscopy analysis.