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ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Biodegradation of Azo Dyes by Three Isolated Bacterial Strains: An Environmental Bioremedial Approach

Maulin P Shah*

Industrial Waste Water Research Laboratory, Applied and Environmental Microbiology Lab, Enviro Technology Limited (CETP), Gujarat, India

*Corresponding Author:
Maulin P Shah
Industrial Waste Water Research Laboratory
Applied and Environmental Microbiology Lab
Enviro Technology Limited (CETP)
Plot No: 2413/2414, GIDC
Ankleshwar- 393 002, Gujarat, India
Tel: +91-90 999 65504
E-mail: [email protected]

Received date: April 03, 2014; Accepted date: July 23, 2014; Published date: July 30, 2014

Citation: Shah MP (2014) Biodegradation of Azo Dyes by Three Isolated Bacterial Strains: An Environmental Bioremedial Approach. J Microbial Biochem Technol S3:007. doi: 10.4172/1948-5948.S3-007

Copyright: © 2014 Shah MP. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

The present study was conducted to investigate the decolorization and degradation of azo dyes using bacteria isolated from textile dye effluent. Three different bacterial species were isolated and the isolates were identified as Bacillus subtilis, Pseudomonas aeruginosa, and Psuedomonas putida. The bacterial inoculums were inoculated into flasks containing azo dyes (500 mg/l) with trace amounts of yeast extract, glucose and sucrose and then sterilized and incubated for 4 days. The decolourization was expressed in terms of percentage decolourization. Psuedomonas putida (95%) was identified as the best decolourizer of Blue RR. Pseudomonas aeruginosa (93%) was the best decolourizer of Black B. The best decolourizer of Red RR was Bacillus subtilis (91%). Bacillus subtillis (65%) highly decolourized the Yellow RR. Pseudomonas aeruginosa (70.58%) was the best decolourizer of Navy Blue. The degradation product after decolorization was examined by thin layer chromatography and Fourier transformed infrared spectroscopy analysis.

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