alexa Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
Open Access

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Research Article

Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii

Irja Sunde Roiha1, Erling Otterlei2 and Ole Bent Samuelsen1,3*

1Institute of Marine Research, P.O. Box 1870 Nordnes, N-5817 Bergen, Norway

2SagaFjord Sea Farm AS, Eldøyane 200, 5411 Stord, Norwaya

3Section of Pharmacology, Institute of Internal Medicine, University of Bergen, Armauer Hansens Hus, 5021 Bergen, Norway

*Corresponding Author:
Dr. Ole Bent Samuelsen
Institute of Marine Research
P.O. Box 1870 Nordnes
N-5817 Bergen, Norway
Fax: +47 55 23 85 31
E-mail: [email protected]

Received Date: May 14, 2010; Accepted Date: June 25, 2010; Published: June 25, 2010

Citation: Roiha IS, Otterlei E, Samuelsen OB (2010) Bioencapsulation of Florfenicol in Brine Shrimp, Artemia Franciscana, Nauplii. J Bioanal Biomed 2:060- 064. doi:10.4172/1948-593X.1000023

Copyright: © 2010 Roiha IS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The brine shrimp Artemia franciscana is one of the most common live-feed organisms for use in the larval culture of marine fi sh production. Bioencapsulation of fl orfenicol, an antibacterial agent, in Artemia nauplii was investigated as a potential carrier for this drug to marine larvae. Florfenicol was delivered directly to the organisms as particles, and the doses ranged from 100 to 2000 mg/l. Analysis of fl orfenicol concentrations in Artemia sp. nauplii were performed using high performance liquid chromatography (HPLC). The uptake of fl orfenicol in Artemia nauplii increased with particle size, dose, and exposure time, obtaining the highest concentration of 5.02 ng/nauplius, using a dose of 300 mg/l AQUAFLOR premix and 10 min exposure time. However, to obtain reproducible results, an enrichment time of at least 60 min is recommended.

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