Biomimetics in Action: Practical Applications of Single Layer Centrifugation for equine breeding
Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, SE-75007 Uppsala, Sweden
- *Corresponding Author:
- Dr. Morrell JM
Swedish University of Agricultural Sciences
Box 7054, SE-75007 Uppsala, Sweden
E-mail: [email protected]
Received date: September 02, 2011; Accepted date: September 20, 2011; Published date: September 26, 2011
Citation: Morrell JM (2011) Biomimetics in Action: Practical Applications of Single Layer Centrifugation for equine breeding. J Veterinar Sci Technol 2:107. doi:10.4172/2157-7579.1000107
Copyright: © 2011 Morrell JM. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Single Layer Centrifugation (SLC) of spermatozoa through a species-specific colloid has been shown to be effective in selecting the best spermatozoa from stallion semen. The method is easier to use and less time-consuming than density gradient centrifugation (DGC), and has been scaled-up to allow whole ejaculates to be processed in a practical manner. The major applications for SLC-sperm selection are as follows: to improve sperm quality in artificial insemination (AI) doses, particularly for “problem” stallions; to increase the “shelf-life” of normal sperm samples, either by processing the fresh semen before preparing AI doses, or by processing the stored semen dose to extract the best spermatozoa; to circumvent the problem of spermatozoa that do not tolerate cooling to 4-6°C; to improve cryosurvival by removing dead and dying spermatozoa prior to cryopreservation, or selecting the live spermatozoa post-thawing; to select morphologically normal spermatozoa with intact chromatin from sub-fertile stallion semen for ICSI, thus increasing the number of blastocysts; to remove pathogens (viruses, bacteria); to accelerate the process of flow cytometric sex selection by removing the dead and dying sperm before passage through the laser beam; to conserve rare breeds. These applications are discussed and practical examples provided.