alexa BIOSYNTHESIS AND CHARACTERIZATION OF SILVER AND IRON NANOPARTICLES FROM SPINACIA OLERACEA AND THEIR ANTIMICROBIAL STUDIES


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Research Article

BIOSYNTHESIS AND CHARACTERIZATION OF SILVER AND IRON NANOPARTICLES FROM SPINACIA OLERACEA AND THEIR ANTIMICROBIAL STUDIES

Karaneh Eftekhari1, K.Mehraj Pasha2, Sanjeeva Prasad Tarigopula3, Mounica Sura4 and Jayasimha Rayalu Daddam4*
  1. Department of Genetics and Biotechnology, Osmania University, Hyderabad, Telangana
  2. Department of Cancer Biology, Dr.Rayalu’s Biotech PVT LTD. Himayath nagar, Hyderabad, Telangana
  3. Department of Zoology, Sri krishnadevaraya University, Anantapur, Andhra Pradesh
  4. Department of Biotechnology, Akshaya Biological Corporation, Himayath Nagar, Hyderabad-29. Telangana
Corresponding Author: Jayasimha Rayalu Daddam, E-mail: [email protected]
Received: 19 October 2014 Accepted: 20 November 2014
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Abstract

Nanoscale Silver and Iron particles have biological applications because of their unique properties, as antimicrobial activity. The aim of the work was to test effects of Silver and Iron nanoparticles from Spinacia oleracea using suggested bacterial colonies. We performed biosafety and biocompatibility tests for Nanoparticles and conjugate with Spinacia oleracea. UV-visible spectroscopy is important technique for analyzing the formation of silver and iron nanoparticles in aqueous solution AgNPs and iron has free electron, which gives rise to plasma resonance absorption band, due combined vibration of metal nanoparticles in resonance with the light wave. The presence of the elemental silver and iron can be seen in the graph presented by EDX, which indicates the reduction of silver ions to elemental silver and iron ions to elemental iron ions. Screening of ethanolic extracts of Spinacia oleracea resulted in moderate antibacterial activities against different bacteria. The silver and iron nanoparticles of average size have been synthesized using dried leaves of plant spinancia oleracea. Characterizations from UV-Vis, SEM, EDX support the stability of biosynthesized nanoparticles. The silver and iron nanoparticles using spinancia proved excellent antibacterial activity.

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