Blue Economy and Biodiversity Surveillance: Fish Caviar Substitute Rapid Discrimination
- *Corresponding Author:
- Gaetano Ciarcia
Interdepartment Research Center for Environment (IRC Env.)
Federico II University, Naples, Italy
E-mail: [email protected]
Received date: August 31, 2015 Accepted date: September 16, 2015 Published date: September 23, 2015
Citation: Trocchia S, Rabbito D, Angelo RD, Ciarcia G, Abdel-Gawad FK, et al. (2015) Blue Economy and Biodiversity Surveillance: Fish Caviar Substitute Rapid Discrimination. J Biodivers Endanger Species S1:002. doi:10.4172/2332-2543.S1-002
Copyright: © 2015 Ciarcia G et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The species identification is a key problem throughout the life cycle of fishes: from eggs and larvae to adults in ecosystem and fisheries research and control, as well as processed fish products labelling in consumer protection. Here, we report a rapid blue biotechnological method applied for egg and fish species discrimination. The amplification of a region of the mitochondrial genome, the cytochrome b, by using the Polymerase Chain Reaction (PCR) permits seafood products species identification. The obtained PCR-products were cut with different restriction endonucleases resulting in species-specific Restriction Fragment Length Polymorphisms (RFLP), which allowed to discriminate, in one step, between different types of caviar substitute species. The following analysis of mitochondrial DNA to control the labelling between caviar substitute species of Cyclopterus lumpus, Mallotus villosus, Trisopterus minutus minutus and the caviar, Acipenser baerii and their monitoring and surveillance is suitable with only one restriction enzyme, MboII. This blue method, applied to a very significant number of samples, retrieved online, allowed a rapid and economic identification of the species, with high percentage of correct identification for fish caviar substitute.