Bovine Viral Diarrhea Milk ELISA Test Detecting Anti-p80 Antibody Association with Milk Handling Methods and Cow Characteristics
David J. Wilson*, Kerry A. Rood and Gregory M. Goodell
Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84341 (Wilson, Rood), the Dairy Authority, Greeley, CO 80634 (Goodell), USA
- *Corresponding Author:
- David J. Wilson
Department of Animal, Dairy, and Veterinary Sciences
Utah State University, Utah Veterinary Diagnostic Laboratory
950 East 1400 North, Logan, UT 84341, USA
Tel: (435) 760-3731
Fax: (435) 797- 2805
E-mail: [email protected]
Received date: March 24, 2012; Accepted date: May 17, 2012; Published date: May 22, 2012
Citation: Wilson DJ, Rood KA, Goodell GM (2012) Bovine Viral Diarrhea Milk ELISA Test Detecting Anti-p80 Antibody – Association with Milk Handling Methods and Cow Characteristics. J Veterinar Sci Technol 3:114.doi:10.4172/2157-7579.1000114
Copyright: © 2012 Wilson DJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A milk ELISA test for Antibody (Ab) against Bovine Viral Diarrhea (BVD) virus was studied in a dairy herd with past diagnoses of calves dying from BVD and Persistently Infected (PI) cows, with culling of all known PI cows. Modified live BVD vaccine was administered to calves 3 months and 4 months old, all cows at dryoff 45 to 60 days before calving, and 15-21 days in milk (DIM). Cows were tested 1 month apart (247 and 258 cows, respectively) using a competitive ELISA for milk Ab binding to p80 BVD non-structural protein. Results are reported as % binding by a second Ab; higher second Ab binding means the milk had less anti-p80 BVD Ab. Cows with 90-100% binding in milk on both tests were classified as low Ab–interpreted as a cow with PI or vaccine failure. Milk handling method was significant; fresh milk mean 49% second Ab binding was higher than for milk preserved 3 other ways. In fresh milk, 15 cows had 90-98% binding on one test, but 14/15 were milking during both herd tests and were below 90% on the other tests. Stage of lactation significantly affected results; anti-BVD Ab was higher from 1-30 DIM and lower from 61-150 DIM than at other stages of lactation. Ear notches were sampled concurrently from all cows for BVD antigen capture ELISA testing. Neither the milk ELISA results (no cows > 90% second Ab binding on both milk tests) nor ear notch testing classified any cows as PI animals. The milk BVD test might be useful to the dairy industry as a practical and convenient test for screening herd replacements, especially when large numbers of lactating cows are purchased and mixed into different pens throughout a dairy herd.