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Bovine Viral Diarrhea Milk ELISA Test Detecting Anti-p80 Antibody Association with Milk Handling Methods and Cow Characteristics | OMICS International | Abstract
ISSN: 2157-7579

Journal of Veterinary Science & Technology
Open Access

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Research Article

Bovine Viral Diarrhea Milk ELISA Test Detecting Anti-p80 Antibody Association with Milk Handling Methods and Cow Characteristics

David J. Wilson*, Kerry A. Rood and Gregory M. Goodell

Department of Animal, Dairy, and Veterinary Sciences, Utah State University, Logan, UT 84341 (Wilson, Rood), the Dairy Authority, Greeley, CO 80634 (Goodell), USA

*Corresponding Author:
David J. Wilson
Department of Animal, Dairy, and Veterinary Sciences
Utah State University, Utah Veterinary Diagnostic Laboratory
950 East 1400 North, Logan, UT 84341, USA
Tel: (435) 760-3731
Fax: (435) 797- 2805
E-mail: [email protected]

Received date: March 24, 2012; Accepted date: May 17, 2012; Published date: May 22, 2012

Citation: Wilson DJ, Rood KA, Goodell GM (2012) Bovine Viral Diarrhea Milk ELISA Test Detecting Anti-p80 Antibody – Association with Milk Handling Methods and Cow Characteristics. J Veterinar Sci Technol 3:114.doi:10.4172/2157-7579.1000114

Copyright: © 2012 Wilson DJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A milk ELISA test for Antibody (Ab) against Bovine Viral Diarrhea (BVD) virus was studied in a dairy herd with past diagnoses of calves dying from BVD and Persistently Infected (PI) cows, with culling of all known PI cows. Modified live BVD vaccine was administered to calves 3 months and 4 months old, all cows at dryoff 45 to 60 days before calving, and 15-21 days in milk (DIM). Cows were tested 1 month apart (247 and 258 cows, respectively) using a competitive ELISA for milk Ab binding to p80 BVD non-structural protein. Results are reported as % binding by a second Ab; higher second Ab binding means the milk had less anti-p80 BVD Ab. Cows with 90-100% binding in milk on both tests were classified as low Ab–interpreted as a cow with PI or vaccine failure. Milk handling method was significant; fresh milk mean 49% second Ab binding was higher than for milk preserved 3 other ways. In fresh milk, 15 cows had 90-98% binding on one test, but 14/15 were milking during both herd tests and were below 90% on the other tests. Stage of lactation significantly affected results; anti-BVD Ab was higher from 1-30 DIM and lower from 61-150 DIM than at other stages of lactation. Ear notches were sampled concurrently from all cows for BVD antigen capture ELISA testing. Neither the milk ELISA results (no cows > 90% second Ab binding on both milk tests) nor ear notch testing classified any cows as PI animals. The milk BVD test might be useful to the dairy industry as a practical and convenient test for screening herd replacements, especially when large numbers of lactating cows are purchased and mixed into different pens throughout a dairy herd.

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