alexa Bovine-Specific Nucleotide Polymorphisms and mRNA Expression of the Growth Hormone Secretagogue Receptor 1a (GHSR1a) Gene and its Genetic Association with Growth and Carcass Traits | Abstract
ISSN: 1747-0862

Journal of Molecular and Genetic Medicine
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Mini Review

Bovine-Specific Nucleotide Polymorphisms and mRNA Expression of the Growth Hormone Secretagogue Receptor 1a (GHSR1a) Gene and its Genetic Association with Growth and Carcass Traits

Masanori Komatsu1*, Yoichi Sato2, Yuki Fujimori3, Tomohito Itoh4, Masahiro Satoh1, Motohide Nishio1, Osamu Sasaki1, Hideaki Takahashi1and Aduli EO Malau-Aduli5

1NARO Institute of Livestock and Grassland Science, National Agriculture and Food Research Organization (NARO), Tsukuba, Ibaraki, Japan

2Animal Industry Research Institute, Iwate Prefecture Livestock Research Centre, Takizawa, Japan

3Nagano Prefecture Livestock Research Centre, Shiojiri, Japan

4Maebashi Institute of Animal Science, Livestock Improvement Association of Japan, Inc. (LIAJ), Maebashi, Japan

5Animal Science and Genetics, Tasmanian Institute of Agriculture, School of Land and Food, University of Tasmania, Hobart, Tasmania, and School of Veterinary& Biomedical Sciences, Faculty of Medicine, Health & Molecular Sciences, James Cook University, Townsville, Queensland, Australia

Corresponding Author:
Masanori Komatsu
Animal Breeding and Genetics Research, Group
NARO Institute of Livestock and Grassland Science
National Agriculture and, Food Research Organization (NARO)
Tsukuba, Ibaraki 305-0901, Japan
Tel: (+81)-29-838-8614
Fax: (+81)-29-838-8623
E-mail: [email protected]

Received date: March 20, 2014; Accepted date: June 12, 2014; Published date: June 20, 2014

Citation: Komatsu M, Sato Y, Fujimori Y, Itoh T, Satoh M, et al. (2014) Bovine-Specific Nucleotide Polymorphisms and mRNA Expression of the Growth Hormone Secretagogue Receptor 1a (GHSR1a) Gene and its Genetic Association with Growth and Carcass Traits. J Mol Genet Med 8:110. doi: 10.4172/1747-0862.1000110

Copyright: © 2014 Komatsu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author(s) and source are credited.

Abstract

The growth hormone secretagogue receptor 1a (GHSR1a) is involved in many important functions including growth hormone (GH) secretion and appetite regulation and other important functions. We reveal herein, the unravelling of bovine-specific 5’untranslated region (5’UTR) microsatellite polymorphisms, a 3bp-indel in exon 1 (DelR242) and two different kinds of transcripts of the GHSR1a gene (spliced, without a microsatellite with in the 5’UTR (GHSR1a); and non-spliced, with the microsatellite (GHSR1b)). A number of 17 alleles ((TG)10~33 ) in the 5’UTR microsatellite was found in 11 cattle breeds. Furthermore, we found the DelR242 (3R) allele, a truncated 3- arginine residue (3R) (major type: 4 arginine residues (4R)) within the intracellular loop 3 of GHSR1a protein in Japanese Shorthorn with a high frequency of 0.43 compared to the low frequency of 0.00~0.09 in other cattle breeds. We carried out a genetic association study between the 5’UTR microsatellite and growth and carcass traits in 1,285 steers. Statistical analysis revealed that the 5’UTR microsatellite locus had a significant additive effect on carcass weight (CW) and average daily gain (ADG). The 19-TG allele had a significantly desirable effect on these traits. We proposed a translational hypothesis that the association is due to differences in the secondary structure of GHSR1b mRNA among the GHSR1a gene haplotypes. We also examined age-related changes in the expressions of GHSR1a and GHSR1b in many cattle tissues. The GHSR1a mRNA expression in the arcuate nucleus of postweaning calves was more than 10-fold higher than those of pre-weaning calves and cows. In peripheral tissues, there were 3 marked differences in mRNA expression between cattle, humans and mice, as follows: (1) the GHSR1a mRNA expression in the liver is high in cattle and very low in humans and mice; (2) the GHSR1b mRNA expression in the liver is low in cattle and high in humans; (3) the GHSR1b mRNA expression in the pancreas is very high in cattle.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version