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ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
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Research Article

C1-Esterase Inhibitor from Human Plasma-An Improved Process to Achieve Therapeutic Grade Purity

Nuvula Ashok Kumar*, Korla Lakshmana Rao, Archana Giri and Komath Uma Devi
Centre for Biotechnology, JNTUH, Hyderabad, India
Corresponding Author : Ashok Kumar N
Centre for Biotechnology, JNTUH
Hyderabad, Telangana, India
Tel: 91-986-669-6098
E-mail: [email protected]
Received August 23, 2014; Accepted September 12, 2014; Published September 17, 2014
Citation: Kumar NA, Rao KL, Archana G, Devi KU (2014) C1-Esterase Inhibitor from Human Plasma-An Improved Process to Achieve Therapeutic Grade Purity. J Bioprocess Biotech 4:174. doi: 10.4172/2155-9821.1000174
Copyright: © 2014 Kumar NA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

C1-Esterase Inhibitor (C1-INH) is a protein present in human plasma and is a member of serine protease inhibitors super family. C1-INH is a molecule with lot of therapeutic importance and is widely used in the treatment of Angioedema and in the current research an improved purification scheme is developed to fractionate C1-INH from human plasma. By a series of chromatography steps C1-INH is purified to homogeneity. A simple three step chromatography procedure has been described excluding the more inefficient or cumbersome methods like precipitation or affinity capture. The C1-INH purified by this scheme is characterized along with the reference molecule. C1-INH produced by this method is a single-chain glycoprotein with a molecular weight of 85-93 KDa with increased purity and yields of 100-120 mg/L of plasma. The chromatography steps are easily scalable and the process is economical and adaptable for therapeutic protein manufacture.

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