alexa Caenorhabditis elegans Model to Test the Effect of Pharmacological Drugs on IGF-1/insulin Signalling Pathway | OMICS International
ISSN: 2155-6156

Journal of Diabetes & Metabolism
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Research Article

Caenorhabditis elegans Model to Test the Effect of Pharmacological Drugs on IGF-1/insulin Signalling Pathway

Jitendra Kumar1,3*#, Anjali Awasthi2#, Kyung-Chae Park3,4, Vijay Kumar Singh5 and Birendra Prasad6

1DBT-PU-IPLS Programme, Department of Botany/Biotechnology, Patna University, Patna- 800005, Bihar, India

2Department of Biology, School of Engineering, Presidency University Bangalore, 560 089, Karnataka, India

3The Buck Institute for Research on Aging, Novato, CA,USA

4Department of Family Medicine, CHA Bundang Medical Center, CHA University, Bundang-gu, Seongnam, Korea

5Central University of South Bihar, Centre for Biological Science (Bioinformatics), Patna- 800014, Bihar, India

6Department of Botany/Biotechnology, Patna University, Patna- 800005, Bihar, India

#Both the authors contributed equally

*Corresponding Author:
Jitendra Kumar
Department of Botany/Biotechnology
Patna University, Patna- 800005, Bihar, India
Tel: +91-99344-84818
E-mail: [email protected]

Received date: October 01, 2015; Accepted date: November 12, 2015; Published date: November 18, 2015

Citation: Kumar J, Awasthi A, Park KC, Singh VK, Prasad B (2015) Caenorhabditis elegans Model to Test the Effect of Pharmacological Drugs on IGF-1/insulin Signalling Pathway. J Diabetes Metab 6:625. doi:10.4172/2155-6156.1000625

Copyright: © 2015 Kumar J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Many pharmacological drugs have been reported to alter insulin signalling in the body resulting in altered blood glucose levels. Drug induced hypoglycaemic or hyperglycaemic effect may lead to adverse effects especially in diabetic patients. Treating ailments of diabetic patients has always remained challenging for the clinicians due to unexplored effect of many drugs on insulin signalling. Insulin/insulin like growth factor-1 signalling (IIS) pathway is highly conserved between Caenorhabditis elegans and humans. In both C. elegans and humans IIS pathway is involved in regulating fat storage. C. elegans dauer formation is regulated primarily via IIS pathway and is triggered by adverse environmental conditions. In this paper we proposed the use of C. elegans dauer formation as a vital strategy to check the drug interaction with IIS. Activity of DAF-2 and DAF-16 are the key regulators of IIS in C. elegans. Aspirin, silymarin and pravastatin drugs have been reported to alter blood glucose levels using animal models and clinical reports. To test the efficacy of our model we tested the effect of these drugs on IIS by using dauer formation as a read-out. Our results report that aspirin and silymarin decreased dauer formation whereas pravastatin enhanced it; the effect was mediated through daf-16 signalling. Our results thus report that C. elegans dauer formation can be used as an effective readout for drug and IIS pathway interaction.

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