Callus Induction and Regeneration Capabilities of Indica Rice Cultivars to Salt StressSamar SR Sankepally1, Venkateswar R Talluri2,3, John P Arulmarianathan3 and Bharat Singh1*
- *Corresponding Author:
- Bharat Singh
Institute of Biotechnology
Amity University Rajasthan
Jaipur 303002, India
Tel: +91 1426 212139
Fax: +91 1426 222836; E-mail: [email protected]
Received date: November 11, 2015 Accepted date: December 23, 2015 Published date: January 06, 2016
Citation: Sankepally SSR, Talluri VR, Arulmarianathan JP, Singh B (2016) Callus Induction and Regeneration Capabilities of Indica Rice Cultivars to Salt Stress. J Biomol Res Ther 4:136. doi:10.4172/2167-7956.1000136
Copyright: © 2016 Sankepally SSR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The main aim of this study was to test the potentiality of PR-115, PR-116, Sambha Mahsuri, Erramallelu, Cotton Dora Sannalu and Pothana local rice cultivars to NaCl stress. Different concentrations and combinations of growth regulators were supplemented in MS media to achieve the callus induction and regeneration in rice cultivars. Maximum frequency of callus induction (75.54 ± 0.91%) was observed in Sambha mahsuri cultivar with MS+2, 4-D (3 mg/L) supplementation but, the response of 2, 4-D (5.0 mg/L) in callus induction was moderate (70.89 ± 0.45%) in Pothana cultivar of indica rice. The highest frequency of regeneration was achieved (65.45 ± 0.51%) in Sambha mahsuri while lowest in Pothana (42.38 ± 0.73%) cultivar of indica rice at 50 mM NaCl concentration. From the results, it has been established that Sambha Mahsuri was more tolerant to salinity in tissue cultures studies (to 50 mM NaCl concentration) so, this cultivar may be used for better production of rice in saline conditions.