alexa Calorimetric Study on the Binding of Lysozyme Upon Interaction With and#946;-Cyclodextrin | OMICS International| Abstract

ISSN: 2157-7544

Journal of Thermodynamics & Catalysis

  • Research Article   
  • J Thermodyn Catal 2010, Vol 1(1): 103
  • DOI: 10.4172/2157-7544.1000103

Calorimetric Study on the Binding of Lysozyme Upon Interaction With β-Cyclodextrin

G Rezaei Behbehani1*, A Taherkhani2,3, A A Saboury4 and A A Divsalar4,5
1Chemistry Department, faculty of science, Islamic Azad University, Takestan branch, Takestan, Iran
2Member of Young Researchers Club, Islamic Azad University, Takestan Branch, Takestan, Iran
3Department of Physics, Islamic Azad University, Takestan Branch, Takestan, Iran
4Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran
5Department of Biological Sciences, Tarbiat Moallem University, Tehran, Iran
*Corresponding Author : G Rezaei Behbehani, Chemistry Department, faculty of science, Islamic Azad University, Takestan Branch, Takestan, Iran, Email: [email protected]

Received Date: Nov 26, 2010 / Accepted Date: Dec 29, 2010 / Published Date: Dec 31, 2010

Abstract

Effects of ?-cyclodextrin, ?CD, on refolding of lysozyme was investigated at pH 12 employing isothermal titration calorimetry (ITC) at 300K in 30mM Tris buffer solution. ?CD was employed as an anti-aggregation agent and the heats obtained for lysozyme+?CD interactions are reported and analyzed in terms of the extended solvation model. It was indicated that there are two sets of identical and non-cooperative sites for ?CD.

Keywords: Lysozyme; Isothermal titration calorimetry; β-cyclodextrin; Binding parameters.

Citation: Behbehani GR, Taherkhani A, Saboury AA, Divsalar A A (2010) Calorimetric Study on the Binding of Lysozyme Upon Interaction With β-Cyclodextrin. J Thermodyn Catal 1: 103. Doi: 10.4172/2157-7544.1000103

Copyright: © 2010 Broadley KJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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