Caspase-12 as a Biomarker of Aquatic Pollution in Oreochromis niloticusSoha M Hamdy1, Amany M Shaban1*, Amany A Mahmoud2, Nahed S Gad3 and Wael A Ibrahim1
- Corresponding Author:
- Amany M Shaban
Faculty of Science, Fayoum University, Egypt
E-mail: [email protected]
Received Date: September 22, 2016; Accepted Date: October 15, 2016; Published Date: October 22, 2016
Citation: Hamdy SM, Shaban AM, Mahmoud AA, Gad NS, Ibrahim WA (2016) Caspase-12 as a Biomarker of Aquatic Pollution in Oreochromis niloticus. J Oceanogr Mar Res 4:149.
Copyright: © 2016 Hamdy SM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.
Biomarkers have recently been used in studies of aquatic environment. Here we present data on stress related bio-markers in Oreochromis niloticus (Nile tilapia) as indicators of aquatic pollution. The samples of study were collected from contaminated aquatic habitats (Qaroun Lake and its neighboring fish farm), in comparison with the reference site. Heavy metal concentrations as Fe, Mn, Zn, Cu and Cd were determined in the water and the fish tissues. Oxidative stress biomarkers (Malondialdehyde, reduced glutathione, glutathione S transferase and catalase) together with endoplasmic reticulum stress biomarker (Caspase-12) were determined in both the blasma and the tissue samples. Histopathological examination of kidney and muscles with SDS electrophoresis of muscle proteins were used to study the influence of pollution on Nile tilapia. Heavy metal concentrations of water and tissue samples from contaminated habitats were significantly higher than the reference site. A highly significant difference in stress related biomarkers was recorded in the fish from contaminated habitats. Histopathological modulations and disrepairs were observed in fish tissues collected from contaminated habitats. Results have shown differences in banding pattern of muscle proteins of Nile tilapia collected from polluted sites. These results provide evidence that caspase-12 is a useful biomarker for aquatic pollutions.