alexa CD44-Positive Cells and Hyaluronan are a Hallmark of a
ISSN: 2157-7099

Journal of Cytology & Histology
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Research Article

CD44-Positive Cells and Hyaluronan are a Hallmark of a Rat Model of Aristolochic Acid Nephropathy

Anne-Emilie Decleves1,2*, Agnieszka Pozdzik2, T Baudoux2, Isabelle Habsch1, Eric De Prez2, Bruno Flamion1, Joelle Nortier2 and Nathalie Caron1

1Molecular Physiology Research Unit, University of Namur, Belgium

2Laboratory of Experimental Nephrology, Faculty of Medicine, Université Libre de Bruxelles, (ULB), Belgium

*Corresponding Author:
Anne-Emilie Decleves
Laboratory of General Physiology
Molecular Physiology Research Unit (URPHYM) University of Namur 61
rue de Bruxelles,B-5000, Belgium
Tel: +32-81-724330
E-mail: [email protected]

Received date: June 12, 2013; Accepted date: August 30, 2013; Published date: September 02, 2013

Citation: Decleves AE, Pozdzik A, Baudoux T, Habsch I, De Prez E, et al. (2013) CD44-Positive Cells and Hyaluronan are a Hallmark of a Rat Model of Aristolochic Acid Nephropathy. J Cytol Histol 4:186. doi:10.4172/2157-7099.1000186

Copyright: © 2013 Decleves AE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Background: Aristolochic acid (AA) Nephropathy (AAN) is characterized by tubulointerstitial injury leading to fibrosis and tubular atrophy. Defective regeneration of tubular cells was hypothesized as a mechanism leading to Proximal Tubular Epithelial Cell (PTEC) atrophy. Here, we examined the distribution of CD44, an adhesion molecule involved in differentiation, and its main ligand, Hyaluronan (HA), in an experimental AAN model.

Methods: Wistar rats were injected daily with AA or vehicle during 35 days. Tubular enzymuria and tissue expression of CD44 and HA were evaluated at days 3, 7, 10, 21 and 35. Co-expressions of CD44 and PCNA (proliferation), vimentin (mesenchymal phenotype), a-SMA (myofibroblasts), and CD133 (progenitor cells) were investigated.

Results: AA induced acute tubular damage followed by PTEC atrophy. In controls, CD44 was limited to the basolateral membrane of collecting ducts while HA expression was confined to the medullary interstitium. CD44 was overexpressed as soon as 3 days after AA exposure, spreading to the apical and basolateral membranes of dedifferentiated PTECs and appearing on numerous interstitial cells along with marked HA accumulation around necrotic tubules. Both CD44 and HA expressions increased steadily throughout the 1-month follow-up. A large amount of tubular and interstitial CD44+ cells were proliferative (PCNA+) and mesenchymal-like (vimentin+). Few CD44+ cells expressed a-SMA or CD133.

Conclusions: Our results suggest CD44 is a strong marker of dedifferentiation, and thus of regeneration, in a rat model of AAN. Concomitant HA accumulation around necrotic tubules may create a sustained crosstalk between CD44+ cells and their interstitial ligand. PTEC atrophy is accompanied by overexpression, not deficiency, of the CD44-HA axis.

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