Cells Harvesting of Tropic Ocean Oleaginous Microalga Strain Desmodesmus Sp. WC08Sen Zhang1,3, Ping-huai Liu2*, Jiang-wei Wu2 and Qing Wang2
- Corresponding Author:
- Ping-huai Liu
College of Materials and Chemical Engineering
Hainan University, Haikou
570228, P.R. China
Tel: +86 0898 66291892
Fax: +86 0898 66291892
E-mail: [email protected]
Received date: June 10, 2015; Accepted date: September 15, 2015; Published date: September 22, 2015
Citation: Zhang S, Liu Ph, Wu Jw, Wang Q (2015) Cells Harvesting of Tropic Ocean Oleaginous Microalga Strain Desmodesmus Sp. WC08. J Fundam Renewable Energy Appl 5:184. doi:10.4172/2090-4541.1000184
Copyright: © 2015 Zhang S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
For biomass recovery of tropic ocean oleaginous microalgae strain Desmodesmus sp. WC08, eight flocculation methods (pH adjustment, Al2 (SO4)3, polyacrylamide, AlCl3, Ca (OH) 2, FeCl3, alum and chitosan) were evaluated and optimized. The results indicated that ferric chloride, aluminum sulfate, aluminum chloride and chitosan exhibited high flocculation ability and their flocculation efficiency were all beyond 94% at the optimal dosage (0.15, 0.4 and 0.03 gL-1, respectively). Chitosan displayed the most tremendous potential for biomass recovery from culture broth based on its feasibility and safety. Acetic acid and hydrous chloride, used to dissolving chitosan, had no significant difference in the flocculation efficiency. And when the pH of culture broth was set 5 or 6, the flocculation efficiency of chitosan was higher and the required flocculation time was less. More dose chitosan was needed to harvest the biomass following the cell growth stage. Overall, the optimal flocculation reagent for harvesting biomass of Desmodesmus sp. WC08 is chitosan, and its optimal flocculation conditions are: just when the pH of the culture broth was set to 6 and the dosage of chitosan was 0.03 g/L at the end of microalgae cultivation, more than 110g of algal biomass can be recovered just by per 1 g of chitosan. Meanwhile, there is little residual chitosan found in the final flocculation supernatant which can be reused to some extent.