Reach Us +44-1647-403003
Cellular Responses to the DNA Damaging Natural Compound Leinamycin | OMICS International | Abstract
ISSN: 1948-5956

Journal of Cancer Science & Therapy
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Cellular Responses to the DNA Damaging Natural Compound Leinamycin

Parvathi Sinha1, YoonJoo Shin1, Allison M. Hays1, Kent Gates2and Daekyu Sun1*

1Department of Pharmacology and Toxicology, College of Pharmacy, University of Arizona, Tucson, AZ 85721, USA

2Departments of Chemistry and Biochemistry, University of Missouri, Columbia, MO 65211, USA

*Corresponding Author:
Daekyu Sun, PhD
Department of Pharmacology and Toxicology
College of Pharmacy, University of Arizona
BIO5 Institute, Room 102,1657 E. Helen Street
Tucson, Arizona 85721, USA
Tel: (520) 626-0323
Fax: (520) 626-4824
E-mail: [email protected]

Received Date: March 28, 2012; Accepted Date: May 09, 2012; Published Date: May 11, 2012

Citation: Sinha P, Shin Y, Hays AM, Gates K, Sun D (2012) Cellular Responses to the DNA Damaging Natural Compound Leinamycin. J Cancer Sci Ther S8:003. doi: 10.4172/1948-5956.S8-003

Copyright: © 2012 Sinha P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Leinamycin is a thiol dependent DNA alkylating agent which shows very potent activity against various human cancer cell lines (IC50 values in the low nanomolar range). This natural compound forms guanine adducts (N7) in DNA which are converted into abasic sites and simultaneously generates Reactive Oxygen Species (ROS), to produce DNA strand breaks in human cancer cells. Our present study shows that leinamycin induces a group of DNA repair and transcription factor genes involved in DNA repair in a MDA-MB-231 human breast cancer cell line, which can mediate chemoresistance to leinamycin. In addition, N-acetylcysteine decreases leinamycin-mediated ROS production while increasing leinamycin mediated apoptotic cell death, without affecting the induction of repair genes. These data indicate that ROS is not a crucial player in leinamycin induced DNA damage and that a precursor of glutathione, N-acetylcysteine, can potentiate leinamycin mediated cytotoxicity by increasing the activation of leinamycin into its DNA reactive form



Share This Page