Cerastes cerastes and Vipera lebetina Snake Venoms Apoptotic Ã¢ÂÂ Stimulating Activity to Human Breast Cancer Cells and Related Gene ModulationShebl RI1, Mohamed AF1, Ali AE2* and Amin MA2
- *Corresponding Author:
- Ali AE
Department of Microbiology & Immunology
Faculty of Pharmacy
Cairo University, Kasr El-Aini St. 11562
Tel: 202 23639307
Fax: 202 23628426
E-mail: [email protected]
Received date: August 10, 2012; Accepted date: September 12, 2012; Published date: September 14, 2012
Citation: Shebl RI, Mohamed AF, Ali AE, Amin MA (2012) Cerastes cerastes and Vipera lebetina Snake Venoms Apoptotic – Stimulating Activity to Human Breast Cancer Cells and Related Gene Modulation. J Cancer Sci Ther 4: 317-323. doi: 10.4172/1948-5956.1000161
Copyright: © 2012 Shebl RI, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Apoptosis occurs normally during development and aging as a homeostatic mechanism to maintain cell populations. Dysregulation of apoptosis can disrupt the equilibrium between cell growth and cell death leading to the development of cancer. Thus, the investigation of new biological apoptotic activators could play an important role in cancer therapy. In the present study, Cerastes cerastes and Vipera lebetina snake venoms were evaluated for their ability to activate apoptosis in cancer cells where test venoms exhibited a concentration and time dependent cytotoxic effect on breast cancer (MCF-7) cells. Typical apoptotic morphological features were demonstrated in venom treated cells detected via transmission electron microscope. In addition, flow cytometric analysis showed an increase in the percentage of apoptotic cells post 24 h treatment relative to venom concentrations. At the molecular level, test venoms induced apoptosis were mediated by up regulation of pro-apoptotic genes (p53 & Bax) and down regulation of anti-apoptotic gene (Bcl-2) in MCF-7 cells, indicating that these venoms could serve as apoptotic stimulators, presenting a novel and potential therapeutic strategy for cancer treatment.