alexa Challenges of Microarray Applications for Microbial Detection and Gene Expression Profiling in Food | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Review Article

Challenges of Microarray Applications for Microbial Detection and Gene Expression Profiling in Food

Yanhong Liu1*, Yan Wang2 and Jie Gang3

1Molecular Characterization of Foodborne Pathogen Research Unit, Eastern Regional Research Center, 600 East Mermaid Lane,Wyndmoor, PA 19038, USA

2Science Research Center, Harbin Institute of Technology, Harbin, Heilongjiang 150001, P. R. China

3Food Engineering Department, College of Life Sciences, Dalian Nationalities University, Dalian, 116600, P. R. China

*Corresponding Author:
Yanhong Liu
Molecular Characterization of Foodborne Pathogen Research Unit
Eastern Regional Research Center
600 East Mermaid Lane
Wyndmoor, PA 19038
Tel: 215-233-6587
Fax: 215-233-6581
E-mail: [email protected]

Received date: April 29, 2011; Accepted June 18, 2011; Published date: June 20, 2011

Citation: Liu Y, Wang Y, Gang J (2011) Challenges of microarray applications for microbial detection and gene expression profiling in food. J Microbial Biochem Technol S2:001. doi:10.4172/1948-5948.S2-001

Copyright: © 2011 Liu Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Microarrays are a high throughput technology used in molecular biology. The different types of microarrays have been applied to clinical and environmental microbiology, microbial ecology, human, veterinary, and plant diagnostics. Since multiple genes can be analyzed simultaneously, this technology has been extended to food microbiology for detection and gene expression analyses of food-borne pathogens. Although this technique has multiple applications, microarray technology presently has low sensitivity and also suffers from problems such as reproducibility and reliability. This paper focuses on microarray applications for microbial detection and gene expression profiling of food-borne pathogens including some of the challenges and key issues such as target nucleic acid isolation and selection of target DNA sequences.

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