alexa Characterisation and Structural Dynamics of Differentially Expressed Proteins of Probiotic Eschericia coli Nissle 1917 in Response to Cocos nucifera Sap | Abstract
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
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Research Article

Characterisation and Structural Dynamics of Differentially Expressed Proteins of Probiotic Eschericia coli Nissle 1917 in Response to Cocos nucifera Sap

Chandrasekhar K1, Sreedevi B1, Sreevani S1, Dileep A1, Lebonah D1, Seshapani P2 and Pramoda kumari J1*

1Department of Microbiology, S.V. University, Tirupati- 517502, India

2K.N. Biosciences, Hyderabad, India

*Corresponding Author:
Pramoda Kumari J
Assistant Professor
Department of Microbiology
S.V.University, Tirupati -517502
A.P, India
Tel: 9491166222
E-mail: [email protected]

Received Date: April 14, 2014; Accepted Date: June 27, 2014; Published Date: June 30, 2014

Citation: Chandrasekhar K, Sreedevi B, Sreevani S, Dileep A, Esther Lebonah D, et al. (2014) Characterisation and Structural Dynamics of Differentially Expressed Proteins of Probiotic Escherichia coli Nissle 1917 in Response to Cocos nucifera Sap. J Proteomics Bioinform 7: 179-185. doi: 10.4172/jpb.1000318

Copyright: © 2014 Chandrasekhar K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

The aim of the present study was to evaluate and analyse the expressed protein structures in response to cocoti sap treated probiotic E. coli Nissle 1917 by MALDI-TOF-MS. The expressed protein spots were separated by 2-D gel electrophoresis and spots were digested with trypsin enzyme for MALDI analysis. Based on their massto- charge ratio, the expressed proteins sequences were collected from Mascot search data. The sequence are analysed by phyre-2 software for homology modelling. The predicted structures were also validated by Rampage software. Of the expressed proteins, only five proteins showed good structural validation in sap treated probiotics. It was concluded that the expressed protein profiles were analysed by 2D-PAGE and MALDI to understand the stress mechanisms of cocoti sap in probiotic E. coli Nissle 1917. Stress mechanism induces many proteins they are involved in metabolic process, regulate gene expression, SOS regulation, transferase activity, purine metabolism and other related activities.

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