Characterization and Confirmation of Papaya ringspot virus-W Strain Infecting Trichosanthese cucumerina at Tamil Nadu, India
- *Corresponding Author:
- Kumar S
Department of Plant Pathology
Tamil Nadu Agricultural University
E-mail: [email protected]
Received date: March 15, 2014; Accepted date: May 02, 2014; Published date: May 08, 2014
Citation: Kumar S, Sankarlingam A, Rabindran R (2014) Characterization and Confirmation of Papaya ringspot virus-W Strain Infecting Trichosanthese cucumerina at Tamil Nadu, India. J Plant Pathol Microb 5:225. doi:10.4172/2157-7471.1000225
Copyright: © 2014 Kumar S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Mosaic and leaf blisters symptoms were observed in Trichosanthese cucumerina (Snake gourd) in regions of coimbatore, India. The biological, physical, serological and molecular properties of the virus isolate were studied. Virus isolate produce chlorotic local lesions on Chenopodium amaranticolor and systemic symptoms on all inoculated cucurbitaceous hosts. The particles appear flexuous rod shape of 750×12 nm under electron microscope. Serologically this isolate identified as strain of PRSV. Dilution end point of virus isolate was 10-4. Thermal inactivation point and Longevity in-vitro of the virus isolate was found to be 55°C and 30 h respectively.