Characterization of β-TCP, β-TCMP and BCMP Produced by Hydrolysis
- Corresponding Author:
- Richard RC
Department of Biomaterials and Biomimetics
NYU College of Dentistry - 345 E. 24th Street
New York, NY 10010, USA
E-mail: [email protected]
Received date: June 08, 2013; Accepted date: July 27, 2013; Published date: August 22, 2013
Citation: Richard RC, Dai J, Sader MS, Soares GA, Thiré RMSM (2013) Characterization of β-TCP, β-TCMP and BCMP Produced by Hydrolysis. Bioceram Dev Appl S1:001. doi:10.4172/2090-5025.S1-001
Copyright: © 2013 Richard RC, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Calcium Deficient Apatites (CDA’s) were prepared by hydrolysis method. The CDA’s were filtered, washed and calcined at 950âÂ°C for 11h. X-ray diffraction demonstrated that β-TCP was obtained after calcining Mg-free CDA and β-TCMP or BCMP were obtained after calcining Mg-substituted CDAs, depending on the Mg/Ca molar ratio of the CDA. Physicochemical characterization was also performed by FT-IR spectroscopy and inductive coupled plasma. Phenolphthalein test was performed, in order to investigate the presence of calcium oxide according to the French standard NF S 94-066. SEM images assessed the morphology of the compounds. Cell viability assay (MTT), calcium nodule formation and the expression of alkaline phosphatase (ALP), osteocalcin, TGF-β1 and collagen were performed in MC3T3-E1 cell line. β-TCP, β-TCMP and BCMP obtained from hydrolysis method weren’t toxic and promoted cell proliferation, showing potential value in bone tissue engineering.