Characterization of Carboxylated-SWCNT Based Potentiometric DNA Sensors by Electrochemical Technique and Comparison with Potentiometric Performance
Mamun M A, A. J. Saleh Ahammad*
Department of Chemistry, Jagannath University, Dhaka, Bangladesh
- *Corresponding Author:
- Mamun M A and Saleh Ahammad AJ
Department of Chemistry
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Received Date: May 27, 2014; Accepted Date: July 04, 2014; Published Date: July 10, 2014
Citation: Mamun MA, Saleh Ahammad AJ (2014) Characterization of Carboxylated- SWCNT Based Potentiometric DNA Sensors by Electrochemical Technique and Comparison with Potentiometric Performance. J Biosens Bioelectron 5:157. doi: 10.4172/2155-6210.1000157
Copyright: © 2014 Mamun MA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Aptasensors for detection of thrombin were produced by covalently linking aptamer (ssDNA) to cSWCNT. Those biosensors were fully characterized by cyclic voltammetry to evaluate the electrode surface charge/ligand density. We performed the CV studies of electrostatically bound [Ru(NH3)6]3+ redox markers on aptamer surfaces and calculated aptamer surface charge density from the CV data. Potentiometric detection of thrombin allows then the correlation with the CV results. The electrode surfaces containing higher amount of aptamers exhibited better performance in potentiometric measurements. These investigations will introduce the pathway to build reusable and regenarable aptasensors including a simple, accurate and precise estimation of aptemer surface charge density to characterize the surface and hence to ensure the quality of apatsensors.