alexa Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

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Research Article

Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells

Marc-Estienne Roehrich1*, Lucio Barile2, Albert Spicher1, Mauro Giacca3 and Giuseppe Vassalli1,2*

1Department of Cardiology, Centre Hospitalier Universitaire Vaudois (CHUV), University of Lausanne, Lausanne, Switzerland

2Molecular Cardiology Laboratory, Fondazione Cardiocentro Ticino, Lugano, Switzerland

3International Center for Genetic Engineering and Biotechnology, Trieste, Italy

*Corresponding Authors:
Giuseppe Vassalli
Cardiology, Department of Cardiology
Centre Hospitalier Universitaire Vaudois (CHUV)
University of Lausanne, Lausanne, Switzerland
E-mail: [email protected]

Marc-Estienne Roehrich
Department of Cardiology
Centre Hospitalier Universitaire Vaudois (CHUV)
University of Lausanne, Lausanne, Switzerland
E-mail: [email protected]

Received date April 30, 2012; Accepted date June 22, 2012; Published date June 24, 2012

Citation: Roehrich ME, Barile L, Spicher A, Giacca M, Vassalli G (2012) Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells. J Stem Cell Res Ther S9:002. doi:10.4172/2157-7633.S9-002

Copyright: © 2012 Roehrich ME, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Cells migrating from heart tissue biopsies during ex vivo culture have been associated with cardiomyogenic and angiogenic potential. We have characterized cells shed by murine cardiac explants cultured in MesenCult MSC medium. A relatively uniform population with a distinguishable morphology emerged from the early cellular outgrowth. This population expressed monocyte/macrophage and hematopoietic markers (CD11b+, CD14+, CD45+) but neither mesenchymal (CD90–, CD105–) nor endothelial (CD31–) markers. It was maintained in culture with a stable phenotype for more than 10 months. When cultured in differentiation medium, cells expressed cardiac sarcomeric α-actinin. They spontaneously formed sphere clusters (“cardiospheres”). This process was enhanced by TNFα. Spheres from neonatal mice exhibited spontaneous beating. To assess whether “contaminating” myocardial tissue fragments would explain CS beating, neonatal Z/EG transgenic mice were injected with an adeno-associated virus (AAV) vector expressing Cre-recombinase from a cardiac-specific promoter (Ncx1), resulting in the excision of a lacZ gene and activatation of the expression of the second reporter gene, enhanced green fluorescent protein (EGFP). Cardiac explants from Z/EG mice receiving AAV-9.Ncx1-CRE expressed EGFP, whereas the cellular outgrowth did not, demonstrating that the presence of cardiomyocytes in the outgrowth was not required for the generation of cardiac progenitor cells. Whether the myelo-monocytoid population originated from a retained hematologic component of explants or cardiac-resident leukocytes remains unclear. These results are in line with recent data on
monocyte-derived multipotent cells (MOMC) with cardiomyogenic potential. They suggest that the cardiac-derived myelo-monocytoid cell, although not considered a classical adult progenitor cell, may have regenerative potential.


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