alexa Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells
ISSN: 2157-7633

Journal of Stem Cell Research & Therapy
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells

Marc-Estienne Roehrich1*, Lucio Barile2, Albert Spicher1, Mauro Giacca3 and Giuseppe Vassalli1,2*

1Department of Cardiology, Centre Hospitalier Universitaire Vaudois (CHUV), University of Lausanne, Lausanne, Switzerland

2Molecular Cardiology Laboratory, Fondazione Cardiocentro Ticino, Lugano, Switzerland

3International Center for Genetic Engineering and Biotechnology, Trieste, Italy

*Corresponding Authors:
Giuseppe Vassalli
Cardiology, Department of Cardiology
Centre Hospitalier Universitaire Vaudois (CHUV)
University of Lausanne, Lausanne, Switzerland
E-mail: [email protected]

Marc-Estienne Roehrich
Department of Cardiology
Centre Hospitalier Universitaire Vaudois (CHUV)
University of Lausanne, Lausanne, Switzerland
E-mail: [email protected]

Received date April 30, 2012; Accepted date June 22, 2012; Published date June 24, 2012

Citation: Roehrich ME, Barile L, Spicher A, Giacca M, Vassalli G (2012) Characterization of Cardiac-derived Myelo-monocytoid Progenitor Cells. J Stem Cell Res Ther S9:002. doi:10.4172/2157-7633.S9-002

Copyright: © 2012 Roehrich ME, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Cells migrating from heart tissue biopsies during ex vivo culture have been associated with cardiomyogenic and angiogenic potential. We have characterized cells shed by murine cardiac explants cultured in MesenCult MSC medium. A relatively uniform population with a distinguishable morphology emerged from the early cellular outgrowth. This population expressed monocyte/macrophage and hematopoietic markers (CD11b+, CD14+, CD45+) but neither mesenchymal (CD90–, CD105–) nor endothelial (CD31–) markers. It was maintained in culture with a stable phenotype for more than 10 months. When cultured in differentiation medium, cells expressed cardiac sarcomeric α-actinin. They spontaneously formed sphere clusters (“cardiospheres”). This process was enhanced by TNFα. Spheres from neonatal mice exhibited spontaneous beating. To assess whether “contaminating” myocardial tissue fragments would explain CS beating, neonatal Z/EG transgenic mice were injected with an adeno-associated virus (AAV) vector expressing Cre-recombinase from a cardiac-specific promoter (Ncx1), resulting in the excision of a lacZ gene and activatation of the expression of the second reporter gene, enhanced green fluorescent protein (EGFP). Cardiac explants from Z/EG mice receiving AAV-9.Ncx1-CRE expressed EGFP, whereas the cellular outgrowth did not, demonstrating that the presence of cardiomyocytes in the outgrowth was not required for the generation of cardiac progenitor cells. Whether the myelo-monocytoid population originated from a retained hematologic component of explants or cardiac-resident leukocytes remains unclear. These results are in line with recent data on
monocyte-derived multipotent cells (MOMC) with cardiomyogenic potential. They suggest that the cardiac-derived myelo-monocytoid cell, although not considered a classical adult progenitor cell, may have regenerative potential.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

mat[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords