alexa Characterization of Egyptian Fluorescent Rhizosphere Ps
ISSN: 2471-2728

Journal of Fertilizers & Pesticides
Open Access

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Research Article

Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita

Khaled Elbanna1*, Hosny Gamal-Eldin1 and Ezzat Abuzaed2

1Department of Agricultural Microbiology, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt

2Department of Plant Protection, Faculty of Agriculture, Fayoum University, Fayoum, 63514, Egypt

*Corresponding Author:
Dr. Khaled Elbanna
Department of Agricultural Microbiology
Faculty of Agriculture, Fayoum University
Fayoum, 63514, Egypt
Tel: +2-084-6380655
Fax: +2-084-6334964
E-mail:
[email protected]

Received date: October 03, 2010; Accepted date: November 08, 2010; Published date: November 10, 2010

Citation: Elbanna K, Gamal-Eldin H, Abuzaed E (2010) Characterization of Egyptian Fluorescent Rhizosphere Pseudomonad Isolates with High Nematicidal Activity against the Plant Parasitic Nematode Meloidogyne Incognita. J Biofertil Biopestici 1:102. doi:10.4172/2155-6202.1000102

Copyright: © 2010 Elbanna K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A total of 52 Egyptian rhizosphere fluorescent pseudomonad isolates (ERFP) were screened for their nematicidal activity in vitro. The screening results showed an inhibitory effect on hatching of Meloidogyne incognita eggs ranging from 57 % to 100 %. Similar to the chemical nematicide Videt in vitro experiment, cultures or cell-free supernatants (CFS) of the ERFP isolates showed complete inhibition of egg hatching and killed 100 % of the J2. In glasshouse pot experiments, cultures of these isolates, CFS and the chemical nematicide showed reduction in root galling ranging from 81.0-95.4, 61.3-84.5, 97.3%, respectively. Also, the reduction in nematode multiplication in soil was 91.9-95.7, 83.5-84.5 and 96.5% for cultures, CFS and the chemical nematicide, respectively, compared to the positive control (nematode only). Furthermore, these isolates showed significant increases in plant growth characters, phenolic content and activities of the plant defense-related enzymes. Based on 16S rDNA sequences and API NE kits, two major clusters were observed; strains Ps 36 and Ps 54 appeared to fall within the variability range of P. putida, while Ps 21, Ps 22 and Ps 14 are likely to be strains of P. aeruginosa. The distinctness of Ps 36 and Ps 54 from Ps 14, Ps 21 and Ps 22 was supported by their physiological characteristics, such as the ability of Ps 14 and Ps 21 and Ps 22 to utilize mannitol, N-acetyl-glucose amine and adipate.

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