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Characterization of Immune Responses to <em>Yersinia pestis</em> (Indian Isolate) Infection in Mouse Model | OMICS International | Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
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Research Article

Characterization of Immune Responses to Yersinia pestis (Indian Isolate) Infection in Mouse Model

Shailendra K Verma1*, Lalit Batra1, Thimmasandra N Athmaram2, Prachi Pathak1, Navya Katram1, Gauri S Agrawal1 and Urmil Tuteja1*
1Microbiology Division, Defence Research & Development Establishment, Jhansi Road, Gwalior-474002, India
2Bioprocess Scale-up Facility, Defence Research & Development Establishment, Jhansi Road, Gwalior-474002, India
Corresponding Authors : Dr. Shailendra K Verma, PhD
Division of Microbiology
DRDE, Jhansi Road
Gwalior-474002, India
Tel: +917512390345
Fax: +917512341148
E-mail: [email protected], [email protected]
  Dr. Urmil Tuteja, PhD
Division of Microbiology, DRDE
Jhansi Road, Gwalior-474002, India
Phone: +917512390330
Fax: +917512341148
E-mail: [email protected]
Received May 27, 2013; Accepted July 01, 2013; Published July 08, 2013
Citation: Verma SK, Batra L, Athmaram TN, Pathak P, Katram N, et al. (2013) Characterization of Immune Responses to Yersinia pestis (Indian Isolate) Infection in Mouse Model. J Clin Cell Immunol 4:151. doi:10.4172/2155-9899.1000151
Copyright: © 2013 Verma SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Yersinia pestis, causative agent of plague, is one of the deadliest pathogens around globe. Innate immune response is first line of host defense against pathogens. Here, we have investigated innate and adaptive immune response in plague infected mice, gene expression levels of TLR1-9 and CD14, MyD88, NF-ĸB, TNF-α, MAPKp38, IL-1β were studied in peritoneal macrophages of plague infected mice in a time dependent manner (0 h, 24 h, 48 h, 72 h, 96 h and 120 h of post infection) by qRT-PCR. We also evaluated the immune response to Yersinia outer proteins (Yops) in Y. pestis infected mice. Selected genes (11 numbers) of Y. pestis encoding virulent factors viz, YpkA, YopH, YopM, V antigen, Pla, YopN, YopJ, YopE, YopK, F1 and pH6 antigen were amplified by PCR, cloned and expressed in Escherichia coli . To study the IgG and its isotypes level, ELISA and immunoblotting were performed using purified recombinant antigens. The major antigens recognized by murine plague infected sera were V antigen, YopH, YopM, YopE, F1 but very weak immunoreaction was observed in case of Pla. We observed a significant difference in IgG isotypes (IgG1, IgG2a, IgG2b and IgG3) to V antigen and F1, whereas in case of YopH and YopE (IgG1 and IgG2b) only. We also observed significant increase in the expression of CD14 at 48 h post infection, TLR4 and MyD88 at 72 h post infection in Y. pestis infected mouse peritoneal macrophages. Our findings suggest that both innate and humoral immune responses are crucial for protection.

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