alexa Characterization of Keratitis Disease Causing 56k Cysteine Protease Encoding Gene from Serratia marcescens | OMICS International | Abstract
ISSN: 0974-276X

Journal of Proteomics & Bioinformatics
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Research Article

Characterization of Keratitis Disease Causing 56k Cysteine Protease Encoding Gene from Serratia marcescens

Anusha Bhaskar*, Akhilesh Upgade and Kavitha P

Centre for Research and Development, PRIST University, Vallam, Thanjavur 614 403, India

*Corresponding Author:
Anusha Bhaskar
Centre for Research and Development
PRIST University, Vallam
Thanjavur 614 403, India
E-mail: [email protected]

Received Date: July 12, 2012; Accepted Date: September 07, 2012; Published Date: September 09, 2012

Citation: Bhaskar A, Upgade A, Kavitha P (2012) Characterization of Keratitis Disease Causing 56k Cysteine Protease Encoding Gene from Serratia marcescens. J Proteomics Bioinform 5: 226-229. doi: 10.4172/jpb.1000241

Copyright: © 2012 Bhaskar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The present study aims at characterization of keratitis causing 56k protease encoding gene, and the homology modeling of this protease from Serratia marcescens. Keratitis is commonly caused by many bacteria such as Staphylococcus aureus, Pseudomonas aeruginosa, Serratia marcescens, viruses and fungi. The aim of the study is to isolate and culture Serratia marcescens, to identify it at the molecular level by amplifying its 16S rDNA and the 56k protease which is thought to be responsible for causing the disease. Both the 16S rDNA and the 56k protease gene were sequenced, and the same deposited in the GenBank with the accession numbers FJ588709 and FJ810078 respectively. The protein 56k protease was modelled and the protein was studied using bioinformatics approach.

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