alexa Characterization of Novel Flavobacterium spp. Involved in the Mortality of Coho Salmon (Oncorhynchuskisutch) in their Early Life Stages
ISSN: 2155-9546

Journal of Aquaculture Research & Development
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Research Article

Characterization of Novel Flavobacterium spp. Involved in the Mortality of Coho Salmon (Oncorhynchuskisutch) in their Early Life Stages

Faisal M1,2* TP Loch1, M Fujimoto3, SA Woodiga3, AE Eissa6, DC Honeyfield4, M Wolgamood5, ED Walker3 and TL Marsh3

1Department of Pathobiology & Diagnostic Investigation, College of Veterinary Medicine, Michigan State University, East Lansing, MI 48824, USA

2Department of Fisheries and Wildlife, College of Agriculture and Natural Resources, Michigan State University, East Lansing, MI 48824, USA

3Department of Microbiology and Molecular Genetics, College of Natural Science, Michigan State University, East Lansing, MI 48824, USA

4US Geological Survey, Northern Appalachian Research Laboratory, 176 Straight Run Road, Wellsboro, Pennsylvania 16901, USA

5Wolf Lake State Fish Hatchery, Michigan Department of Natural Resources, 34270 County Road 652, Mattawan, Michigan 49071, USA

6Department of Fish Diseases and Management, Cairo University, Giza, 12211, Egypt

*Corresponding Author:
Mohamed Faisal
174 Food Safety and Toxicology Building
Michigan State University
East Lansing, MI 48823
Tel: 1-517-884-2019
Fax: 1-517-432-2310
E-mail: [email protected]

Received Date: November 03, 2011; Accepted Date: December 12, 2011; Published Date: December 21, 2011

Citation: Faisal M, TP Loch, M Fujimoto, SA Woodiga, AE Eissa, et al. (2011) Characterization of Novel Flavobacterium spp. Involved in the Mortality of Coho salmon (Oncorhynchus kisutch) in Their Early Life Stages. J Aquac Res Development S2:005. doi:10.4172/2155-9546.S2-005

Copyright: © 2011 Faisal M, et al.This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Salmonid species in the Laurentian Great Lakes basin suffer from early mortalities that are often associated with low survival rates of swim-up fry. Coho salmon (Oncorhynchus kisutch) alevins exhibiting a spinning swimming behavior and convulsions were presented to the Aquatic Animal Health Laboratory of Michigan State University. Twelve yellow-pigmented bacterial isolates that were motile via gliding were recovered from the brains of dead and moribund fish and identified as Flavobacterium spp. (designated CS: 29-31, 34-38, 40-43). 16S rRNA sequence analysis indicated that 9 of the 12 sequences were grouped into a single clade (CS29, 31, 34-38, 40, 41) and were essentially identical over the 676 aligned bases used in the phylogenetic analysis. This clade is closely related to the eel-pathogenic Flavobacterium johnsoniae-like YO60 recovered from South Africa. The remaining three isolates, CS30, CS42, CS43 were closest to Flavobacterium sp. Wuba46 (100%), Flavobacterium sp. EP125 (100%), and Flavobacterium sp. WB 4.3-15 (99.9%), all of which were reported from European waters. Experimental challenge of five month old coho salmon fry via intra-peritoneal injection with the CS36 isolate resulted in morbidity and mortality rates of approximately 10% in the two highest infection doses. Clinical signs included tachybranchia, hemorrhages, and fin erosion. Stained tissue sections from dead and moribund fish showed degeneration of kidney tubules, edema in the renal interstitial tissues, heterophilic cellulitis and myodegenerative changes within the caudal peduncle, and a proteinaceous exudate in the coelomic cavity. Based on this study, flavobacterial infections with these isolates, which were detected for the first time in North America, can potentially cause losses in yolk sac and swim-up coho salmon fry.

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