Characterization of Pseudomonas viridiflava Causing Alfalfa Root Rot Disease in Hamedan Province of Iran
Ali Heydari, Gholam Khodakaramian* and Doostmorad Zafari
Department of Plant Protection, College of Agriculture, Bu-Ali Sina University, Hamedan, Iran
- *Corresponding Author:
- Gholam Khodakaramian
Department of Plant Protection
College of Agriculture
Bu-Ali Sina University
E-mail: [email protected]
Received date: August 16, 2012; Accepted date: September 10, 2012; Published date: September 15, 2012
Citation: Heydari A, Khodakaramian G, Zafari D (2012) Characterization of Pseudomonas Viridiflava Causing Alfalfa Root Rot Disease in Hamedan Province of Iran. J Plant Pathol Microb 3:135. doi:10.4172/2157-7471.1000135
Copyright: © 2012 Heydari A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Alfalfa (Medicago sativa) is the most important forage crop in Hamedan province, located in west of Iran. Under field condition, plants showed growth stunting, chlorosis and wilting symptoms not previously reported. To survey the causal agent of the disease, symptomatic plants were collected from the main alfalfa growing area in this province. Plants samples were disinfected, grinded in phosphate buffer, and a loop full of the prepared suspension was stroked on nutrient agar medium containing 5% yeast extract and 1% glycerol. Isolation and characterization of the causal agent, especially from plants in primary growth stage of the disease, is difficult. Isolated bacteria caused wilting and chlorosis after 40 days on alfalfa plants, under green house condition. Phenotypic feature determination of the isolated bacteria, together with two defined strains, by standard bacteriological methods indicated that they belong to P. viridiflava. PCR assay confirmed results of identification of the causal bacterial agent, determined by classical methods. Tested representative bacterial isolates showed an 860 pb PCR band, using primers designed for 16S rDNA sequence of P. viridiflava LMG 2352T (type strain). This new reported bacterium is the second pathogenic bacterium agent, which causes alfalfa yield losses in Hamedan province of Iran.