alexa Characterization of the Pear (Pyrus ussuriensis) Actin-depolymerizing Factor PuADF During Fruit Ripening
ISSN: 2376-0354

Journal of Horticulture
Open Access

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Characterization of the Pear (Pyrus ussuriensis) Actin-depolymerizing Factor PuADF During Fruit Ripening

Hui Yuan, Zhongyu Jiang, Lichao Zhang, Pengtao Yue and Aide Wang*

College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China

Corresponding Author:
Aide Wang
College of Horticulture, Shenyang Agricultural University, Shenyang, 110866, China
Tel:
+86-24-88340295
Fax:
+86-24-88340295
E-mail:
[email protected]

Received Date: July 22, 2016; Accepted Date: August 30, 2016; Published Date: September 08, 2016

Citation: Yuan H, Jiang Z, Zhang L, Yue P, Wang A (2016) Characterization of the Pear (Pyrus ussuriensis) Actin-depolymerizing Factor PuADF During Fruit Ripening. J Hortic 3:183. doi: 10.4172/2376-0354.1000183

Copyright: © 2016 Yuan H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The polymerization or depolymerization of the cytoskeleton is very important in various aspects of plant development. ADF (actin-depolymerizing factor) is a typical actin binding protein that can mediate the polymerization or depolymerization of the actin cytoskeleton, but its role in fruit ripening remains unclear. Here, we characterized an ADF gene, PuADF. The expression of PuADF was evaluated in young leaves, stems, flowers, and roots as well as in fruits. The expression of PuADF was induced by ethephon treatment but inhibited by 1-MCP treatment. To explore the network of PuADF function in ‘Nanguo’ pear fruits ripening, we screened a cDNA library from ‘Nanguo’ pear fruits using PuADF as bait. Two potential interactors of PuADF were identified, PuAS (Asparagine Synthetase) and PuDAD1 (Defender Against Death 1), both of which have been associated with PCD (Programmed Cell Death). This direct interaction was further confirmed by yeast two-hybrid analyses. The expression levels of PuAS and PuDAD1 were affected by ethylene. Our results indicated that PuADF is involved in ethylene-mediated fruit ripening and might be related to PCD during fruit ripening. The possible mechanisms are discussed.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords