alexa Characterization of the Volatile Components of Cannabis Preparations by Solid-Phase Microextraction Coupled to Headspace-Gas Chromatography with Mass Detector (SPME-HSGC/MS) | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

Characterization of the Volatile Components of Cannabis Preparations by Solid-Phase Microextraction Coupled to Headspace-Gas Chromatography with Mass Detector (SPME-HSGC/MS)

Sebastiano Arnoldi, Gabriella Roda*, Eleonora Casagni, Lucia Dell’Acqua, Michele Dei Cas, Fiorenza Fare, Chiara Rusconi, Giacomo Luca Visconti and Veniero Gambaro

Department of Pharmaceutical Sciences, University of Milan, Via Mangiagalli 25, 20133, Milan, Italy

*Corresponding Author:
Gabriella Roda
Department of Pharmaceutical Sciences
University of Milan, Via Mangiagalli 25
20133, Milan, Italy
Tel: 02503 19328
E-mail: [email protected]

Received date: January 30, 2017; Accepted date: February 02, 2017; Published date: February 08, 2017

Citation: Arnoldi S, Roda G, Casagni E, Dell’Acqua L, Cas MD, et al. (2017) Characterization of the Volatile Components of Cannabis Preparations by Solid-Phase Microextraction Coupled to Headspace-Gas Chromatography with Mass Detector (SPME-HSGC/MS). J Chromatogr Sep Tech 8:350. doi: 10.4172/2157-7064.1000350

Copyright: © 2017 Arnoldi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Solid phase microextraction coupled to headspace sampling and GC/MS technique was applied to the characterization of the volatile components of several Cannabis preparations (hashish). Different parameters of the analytical method (fiber, coating thickness, sampling and exposition temperatures, sample preparation) were evaluated to optimize the characterization of the volatile components. a-Pinene, ß-myrcene, limonene, 4-carene, trans-3(10) caren-2-ol, 4,7,7-trimethylbicyclo [4.1.0] heptan-3-ol, caryophyllene, ß-humulene, azulene, gurjunene, ledene and caryophyllene oxide were identified among the volatile components of all hashish preparations. Moreover, a suitable internal standard (nonane) was chosen, the reproducibility and linearity of the method were evaluated in order to carry out the quantitative determination of caryphyllene, the most abundant volatile terpene. Its quantity ranged from 800 to 3000 µg/g.

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