alexa Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
Open Access

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Research Article

Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode

Yosra Braham1*, Houcine Barhoumi1, Abderrazak Maaref1, Amina Bakhrouf2, Christine Grauby Heywang3, Tauria Cohen Bouhacina3 and Nicole Jaffrezic-Renault4

1Laboratory of Interfaces and Advanced Materials (LIMA), University of Monastir 5000, Tunisia

2Laboratory of Analysis, Treatment and Valorization of Environment Pollutants and Products, Faculty of Pharmcia, University of Monastir 5000 Tunisia

3Laboratory of Waves and Aquitaine Matter CNRS, UMR, 5798, University of Bordeaux 1, 351, crs, Liberation, 33405, Talence, France

4Laboratory of Analytical Sciences, UMR CNRS 5180, University of Claude Bernard-Lyon1, Bâtiment Raulin, 69622 Villeurbanne Cedex, France

*Corresponding Author:
Yosra Braham
Laboratory of Interfaces and
Advanced Materials (LIMA)
University of Monastir 5000, Tunisia
Tel: + (216) 78 500 278
Fax: + (216) 78 500 280
E-mail: [email protected]

Received Date: June 28, 2014; Accepted Date: September 08, 2014; Published Date: February 15, 2015

Citation:Braham Y, Barhoumi H, Maaref A, Bakhrouf A, Heywang CG, et al. (2015) Characterization of Urea Biosensor Based on the Immobilization of Bacteria Proteus Mirabilis in Interaction with Iron Oxide Nanoparticles on Gold Electrode. J Biosens Bioelectron 6:160. doi: 10.4172/2155-6210.1000160

Copyright: © 2015 Braham Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

In this work we describe a new urea biosensor, based on the immobilization of bacteria, Proteus mirabilis on gold electrode. To improve the stability of the bio-system, additional materials were used such as functionalized Fe3O4 nanoparticles (NPs), cationic (PAH), anionic (PSS) polyelectrolytes, Bovine Serum Albumin (BSA) and glutaraldehyde as a cross-linking agent. The electrochemical performances of the developed bacteria biosensor was evaluated using the electrochemical impedance spectroscopy (EIS) and cyclic voltammetry measurements. The adhesion of the bacteria cell on gold electrode was evaluated using contact angle measurements. The morphology of bacteria and its interaction with Fe3O4 nanoparticles were evaluated with the atomic force microscopy (AFM). As a result, a sensitive, stable and reproducible urea biosensor was developed.

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