alexa Characterization of Variable Regions of the Gp120 Protein from HIV-1 Subtype C Virus Variants Obtained from Individuals at Different Disease Stages in Sub-Saharan Africa | Abstract
ISSN 2155-6113

Journal of AIDS & Clinical Research
Open Access

Like us on:

OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Characterization of Variable Regions of the Gp120 Protein from HIV-1 Subtype C Virus Variants Obtained from Individuals at Different Disease Stages in Sub-Saharan Africa

A. Cenci1, L. Tavoschi1, G. D’ Avenio2, P. Narino1, S. Becattini1,6, D. Bernasconi1,7, M. Chiappi1,8, L. La Torre1, H. Sukati3, E. Vardas4, A. Lo Presti5, E Cella5, M. Ciccozzi5, O. Picconi1, P. Monini1, B. Ensoli1 and S. Buttò1*

1Istituto Superiore di Sanità, National AIDS Center, Rome, Italy

2Istituto Superiore di Sanità, Department of Technology and Health, Rome, Italy

3Ministry of Health, National Center Public Health Laboratory, Manzini, Swaziland

4Stellenbosch University, Division of Medical Virology, Stellenbosch, South Africa

5Istituto Superiore di Sanità, Department of Infectious, Parasitic and Immunomediated Diseases, Rome, Italy

6Institute for Research in Biomedicine, Bellinzona, Switzerland

7Abbott diagnostics Italy

8Departamento de Estructura de Macromoléculas, Centro Nacional de Biotecnología, Consejo Superior de Investigaciones Científicas (CNB-CSIC), Madrid, Spain

*Corresponding Author:
Stefano Buttò
National AIDS Center
Istituto Superiore di Sanità
V.le Regina Elena 299, 00161 Rome, Italy
Tel: +39-06-49903249
Fax: +3906-49903002
E-mail: [email protected]

Received Date: April 16, 2012; Accepted Date: May 16, 2012; Published Date: May 20, 2012

Citation: Cenci A, Tavoschi L, D’ Avenio G, Narino P, Becattini S, et al. (2012) Characterization of Variable Regions of the Gp120 Protein from HIV-1 Subtype C Virus Variants Obtained from Individuals at Different Disease Stages in Sub-Saharan Africa. J AIDS Clinic Res S8:006. doi:10.4172/2155-6113.S8-006

Copyright: © 2012 Cenci A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The development of a vaccine against HIV/AIDS capable of preventing virus infection has been hampered by the HIV envelope (Env) heterogeneity that makes it difficult to induce neutralizing antibodies against Env proteins from different HIV clades. Several studies have indicated that gp120 Env protein sequence tends to change considerably during the course of HIV disease which allows the virus to escape the immune responses. In order to define gp120 sequence changes, we have characterized the V1, V2, V4 and V5 variable regions of gp120 variants from 72 HIV-1-clade-C-infected subjects from South Africa and Swaziland, which were naïve to antiretroviral (ARV) therapy and at different disease stages. Sequence characteristics, such as aminoacid sequence length, presence of Putative N-Glycosylation Sites (PNGSs) and electric charge were investigated.

Methods: According to the Avidity Index value and CD4+ T cell count, patients were classified for disease stage in three groups: recent, chronic and late stage, each one comprised of 24 patients. The V1 to V5 Env variable regions were directly PCR amplified from plasma virus RNA and sequenced.

Results: A significant increase in the amino acid sequence length of V1 and V4 domains, and a corresponding increase of the “shifting” PNGSs were observed in the HIV variants obtained from individuals at chronic stage of disease, as compared to the recent infection group. Finally, a significant increase of the net electric positive charge of the V5 loop was found in the HIV variants from the group of subjects with late disease, as compared to the chronic disease group.

Conclusion: We conclude that changes in sequence length, glycosylation pattern and net electrical charge in the variable V1, V4 and V5 regions of gp120 occur in the course of HIV infection, possibly in response to the pressure of the host immune response.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

bornova escort

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version