Chemical Synthesis, Characterization and Bioactivity Evaluation of Hydroxyapatite Prepared from Garden snail (Helix aspersa)
Anjuvan Singh1* and K. M. Purohit2
1Department of Biotechnology, Lovely School of Sciences, Lovely Professional University, Phagwara, India
2Department of Chemistry, National Institute of Technology, Rourkela –769008, Orissa, India
- Corresponding Author:
- Dr. A. Singh
Lecturer, Department of Biotechnology
Lovely School of Sciences Lovely Professional University
E-mail: [email protected]
Received date: April 23, 2011; Accepted date: May 4, 2011; Published date: June 11, 2011
Citation: Singh A, Purohit KM (2010) Chemical Synthesis, Characterization and Bioactivity Evaluation of Hydroxyapatite Prepared from Garden snail (Helix aspersa). J Bioprocess Biotechniq 1:104. doi:10.4172/2155-9821.1000104
Copyright: © 2010 Singh A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A novel attempt is made to convert the calcium carbonate skeleton of widely available garden snail shell (Helix aspersa) to hydroxyapatite based bioceramics. The snail shell was found to decompose within 8500C to all the carbonate phases. The calcined snail shells were then treated with acids followed by different chemicals in ammoniacal media maintaining proper stoichemetry to produce fine Hydroxyapatite (HAP) as filter cake with Ca/P molar ratio of 1.67. The dried HAP powder was extremely pure with specific surface area of 15m2/g. The different characterization techniques were adopted both for calcined snail shell and HAP synthesized like X-ray Diffraction (XRD), Thermal Analysis (DTA/TGA), Fourier Transform Infra red Spectroscopy (FTIR) and Scanning Electron Microscopy (SEM). The surface area and the particle size, of the HAP powder prepared by chemical precipitation route, were also determined by BET and Malvern particle size analyzer respectively. The synthesized powder was soaked in stimulated body fluid (SBF) medium for various periods of time in order to evaluate its bioactivity. The changes of the pH of SBF medium were measured. High bioactivity of prepared HAP powder due to the formation of apatite on its surface was observed.